棉酚对雄性小鼠的生殖毒性作用

发布时间：2018-04-24 13:47

本文选题：棉酚 + 雄性小鼠　；参考：《中国兽医杂志》2015年07期

【摘要】：为了观察棉酚对雄性小鼠的生殖毒性,将48只雄性小鼠随机分成4组。对照组(羧甲基纤维素钠)、低剂量组(30 mg/kg·d)、中剂量组(60 mg/kg·d)和高剂量组(240 mg/kg·d)。连续灌胃给药20 d。第21天处死,取睾丸称重并计算脏器指数,将睾丸进行病理组织学观察。结果:前两周,高剂量组体重显著下降(P0.05),对照组体重显著上升(P0.05),第3周高剂量组体重差异极显著(P0.01);与对照组相比,低剂量组睾丸脏器指数差异极显著(P0.01),中剂量组差异显著(P0.05),高剂量组差异不显著(P0.05);睾丸组织进行H.E.染色,对照组无明显病理变化,低、中剂量组病变轻微,高剂量组正常结构遭到破坏,结构松散。结论:棉酚能够抑制小鼠体重的增长,不同浓度的棉酚对小鼠睾丸组织造成不同程度的病理损伤,60 mg/kg·d和240 mg/kg·d棉酚对雄性小鼠有致死作用。
[Abstract]:In order to observe the reproductive toxicity of gossypol on male mice, 48 male mice were randomly divided into 4 groups. Control group (sodium carboxymethyl cellulose), low dose group (30 mg/kg), middle dose group (60 mg/kg d) and high dose group (240 mg/kg / d). The drug was continuously administered by gavage for 20 days. On the 21st day, the testis were weighed and the viscera index was calculated, and the testis were observed histopathologically. Results: in the first two weeks, the body weight of the high dose group decreased significantly, while that of the control group increased significantly (P 0.05), and the weight difference of the high dose group in the third week was extremely significant (P 0.01), compared with that in the control group. The index of testicular viscera in low dose group was significantly different from that in middle dose group (P 0.05), while in high dose group there was no significant difference (P 0.05). Staining showed that there were no obvious pathological changes in the control group, while in the middle dose group, the pathological changes were slight, while the normal structure in the high dose group was damaged and the structure was loose. Conclusion: gossypol can inhibit the weight gain of mice. Different concentrations of gossypol can cause different degrees of pathological damage to testis of mice. Gossypol for 60 mg/kg d and 240 mg/kg d has lethal effect on male mice.
【作者单位】：东北农业大学动物医学学院;
【分类号】：R114

【参考文献】