锰致神经细胞损伤中诱导型一氧化氮合酶及其基因的变化

发布时间：2018-04-24 18:51

本文选题：锰 + 一氧化氮　；参考：《环境与职业医学》2015年03期

【摘要】：[目的]观察不同浓度锰对大鼠脑片神经细胞的损伤情况,探讨诱导型一氧化氮合酶(i NOS)在锰诱导神经细胞损伤中的变化。[方法]培养出生后4~7 d大鼠脑片,培养液为50%高糖杜尔伯科改良伊格尔培养基,25%Hank平衡盐溶液,24%热灭活马血清,1%青霉素和链霉素。待第15天脑片神经细胞生长状态最佳时加入0、25、100、400μmol/L Mn Cl2。培养24 h后,测定培养液中乳酸脱氢酶(LDH)活性,脑片细胞悬液中细胞凋亡率、一氧化氮生成量和i NOS活性,m RNA和蛋白表达水平。[结果]不同浓度锰处理脑片24 h后,脑组织切片神经细胞发生明显损伤。随着锰处理浓度升高,LDH活性升高,100和400μmol/L锰处理组是对照组的1.71、2.76倍。细胞凋亡率和一氧化氮生成量升高增加,25、100和400μmol/L锰处理组分别是对照组的3.31、4.50和6.97倍和1.98、2.79和4.02倍。i NOS活性增强,100和400μmol/L锰处理组分别是对照组的2.12和2.64倍。i NOS m RNA和蛋白表达水平明显升高,25、100和400μmol/L锰处理组i NOS m RNA表达水平是对照组的1.27、1.43和1.86倍。100和400μmol/L锰处理组i NOS蛋白表达水平分别是对照组的4.17和5.50倍。[结论]锰可致大鼠脑片神经细胞一氧化氮生成量和i NOS表达升高,进一步导致细胞凋亡率增加。
[Abstract]:[objective] to observe the injury of different concentrations of manganese on neurons in rat brain slices, and to investigate the changes of inducible nitric oxide synthase (iNOS) in the injury of neurons induced by manganese. [methods] the rat brain slices were cultured for 4 days after birth. The culture medium was 50% high sugar Duroc modified Eagle medium with 25% Hank equilibrium salt solution and 24% hot inactivated horse serum penicillin and streptomycin. On the 15th day, the best growth state of neurons was obtained by adding 0 ~ 25100,400 渭 mol/L mn Cl _ 2. After 24 h culture, the activity of lactate dehydrogenase (LDH), apoptosis rate, nitric oxide production, activity of I NOS and protein expression were measured. [results] after 24 h treatment with different concentrations of manganese, the nerve cells in brain tissue sections were obviously damaged. With the increase of manganese concentration, the activity of LDH was increased by 1.71 ~ 2.76 times of that of the control group in 100 渭 mol/L and 400 渭 mol/L mn treatment groups. The increase of apoptosis rate and nitric oxide production increased by 3.31 NOS 4.50 and 6.97 times and 1.98 渭 mol/L 2.79 and 4.02 times of the control group respectively. I NOS activity increased 2.12 times and 2.64 times of that of the control group. I NOS m RNA and eggs in the 400 渭 mol/L manganese treatment group were 2.12 and 2.64 times higher than those in the control group, respectively. The expression level of I NOS m RNA in the white cells was significantly higher than that in the control group (1.271.43, 1.86 fold. 100 and 400 渭 mol/L), and the expression of I NOS protein was 4.17 and 5.50 times higher than that in the control group, respectively. [conclusion] Manganese can induce the increase of no production and I NOS expression in rat brain slices and further increase the apoptosis rate.
【作者单位】：中国医科大学附属第一医院药学部;中国医科大学公共卫生学院环境卫生学教研室;
【基金】：国家自然科学基金项目(编号:81102098)
【分类号】：R114

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