ELOVL2基因多态性与乳母乳汁DHA水平的关联性分析

发布时间：2018-04-25 03:32

本文选题：基因多态性 + 单倍型　；参考：《吉林大学学报(医学版)》2015年06期

【摘要】：目的:探讨ELOVL脂肪酸延长酶基因2(ELOVL2)rs2281591和rs3798713位点与乳母乳汁二十二碳六烯酸(DHA)水平的关系,阐明ELOVL2基因多态性对乳汁DHA水平的影响。方法:选取健康产妇209人,在产后第22~25天签署知情同意书并进行三天二十四小时膳食回顾调查,收取乳汁20mL,应用气相色谱法检测乳汁DHA水平,并提取乳汁基因组DNA,应用Sequenom Mass Array系统检测ELOVL2基因2个单核苷酸多态性(SNP)位点基因型,采用UNPHASED 3.012遗传学软件进行多位点单倍型与乳汁DHA水平的数量性状分析。结果:ELOVL2基因rs2281591和rs3798713位点基因型频数分布均符合Hardy-Weinberg平衡定律(P0.05);不同基因型的乳母膳食脂肪酸摄入量和乳汁DHA水平差异均无统计学意义(P0.05);不同rs3798713(CG)-rs2281591(AG)单倍型的乳母乳汁DHA水平比较差异无统计学意义(χ2=3.422,df=5,P=0.635)。结论:ELOVL2基因rs3798713和rs2281591位点及其组成的单倍型与乳母乳汁DHA水平无关联。
[Abstract]:Objective: to investigate the relationship between the 2(ELOVL2)rs2281591 and rs3798713 loci of ELOVL fatty acid prolongation gene and the level of 22 carbohexaenoic acid DHA in breast milk, and to elucidate the effect of ELOVL2 gene polymorphism on the DHA level in milk. Methods: 209healthy puerperas were selected and informed consent was signed on the 22nd and 25th day postpartum, and the 24 hours diet was retrospectively investigated for three days. The milk was collected for 20mL and the DHA level was detected by gas chromatography. The genomic DNA of milk was extracted, and the genotypes of two single nucleotide polymorphisms (SNPs) of ELOVL2 gene were detected by Sequenom Mass Array system, and the quantitative characters of multilocus haplotype and DHA level of milk were analyzed by UNPHASED 3.012 genetic software. Results the frequency distribution of rs2281591 and rs3798713 loci in the rs2281591 and rs3798713 loci of the 10% ELOVL2 gene were in accordance with the Hardy-Weinberg equilibrium law (P0.05A); there was no significant difference in dietary fatty acid intake and DHA level among different genotypes of lactating mothers; and DHA haplotypes of different rs3798713 CG-rs2281591 (AGG) haplotypes were not significantly different. There was no statistical difference between the two groups (蠂 ~ 2 = 3.422 d ~ (fen) ~ (5) P ~ + ~ (0.635)). Conclusion there is no correlation between the rs3798713 and rs2281591 loci and their haplotypes in the rs3798713 and rs2281591 genes of the EloVL2 gene and the level of DHA in breast milk.
【作者单位】：吉林大学公共卫生学院营养与食品卫生教研室;吉林大学公共卫生学院预防医学实验中心;
【基金】：国家自然科学基金资助课题(81102115)
【分类号】：R151.42

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