运动对急性暴露于2,3,7,8-四氯二苯并二恶英大鼠肝组织酶活性的影响

发布时间：2018-04-29 03:15

  本文选题：-四氯二苯并二恶英( + -TCDD)　； 参考：《生态毒理学报》2015年02期

【摘要】：为了研究运动对2,3,7,8-四氯二苯并二恶英(2,3,7,8-TCDD)急性暴露大鼠肝组织酶活性的影响,将40只雄性Wistar大鼠随机分为正常对照组(NC)、染毒组(NT)、运动对照组(EC)、运动染毒组(ET)。染毒组(NT组与ET组)腹腔注射10μg·kg-1(以单位体重计)的TCDD,对照组(NC组与EC组)腹腔注射等量的玉米油;NT、NC组静养4周,ET、EC组运动(尾部负重5%游泳30分钟)4周。4周后,称重并宰杀大鼠,分离肝组织,称重后-80℃保存待测7-乙氧基异吩恶唑酮脱乙基酶(EROD)、7-乙氧基香豆素-O-脱乙基酶(ECOD)及芳香烃羟化酶(AHH)的活性。将数据进行多因素方差分析(MAVONA)处理,结果表明,染毒可降低大鼠体重,增加肝湿重和肝相对重量、增加EROD、ECOD活性;运动可增加大鼠肝相对重量、增加AHH的活性;染毒后运动可降低EROD、ECOD的活性。结论:急性10μg·kg-1(以单位体重计)TCDD染毒后4周可增加大鼠肝相对重量;4周的运动能有效降低TCDD对EROD、ECOD活性的激活作用。
[Abstract]:In order to study the effect of exercise on the activity of hepatic enzyme in rats exposed to acute exposure to TCDD, 40 male Wistar rats were randomly divided into three groups: normal control group, exercise control group, and exercise exposure group. TCDD of 10 渭 g / kg -1 (in unit body weight) was injected intraperitoneally in NT group and et group, and the control group and EC group were injected intraperitoneally with the same amount of corn oil NTN group for 4 weeks. The rats were weighed and slaughtered after 4 weeks of exercise (5% tail weight swimming for 30 minutes and 4 weeks later). The liver tissue was isolated, and the activities of 7-ethoxycoumarin -O-deethylase (ECOD) and aromatics hydroxylase (AHH) were preserved at -80 鈩，

本文编号：1818142