双酚AF胁迫对斑马鱼早期发育相关基因的影响

发布时间：2018-04-30 15:04

本文选题：双酚AF + 斑马鱼　；参考：《生物技术》2017年01期

【摘要】：[目的]探讨双酚AF胁迫对斑马鱼早期发育的影响。[方法]采用半静态染毒的方式暴露受精卵7d(0、5、50和500μg/L),通过显微观察与实时荧光定量PCR方法,检测孵化率及相关基因表达量变化。[结果]50、500μg/L BPAF暴露影响斑马鱼胚胎72 h孵化率,分别下降44.97%、49.44%;下丘脑trh基因在各暴露组无明显变化,而下丘脑crh基因在胚胎(幼鱼)期各暴露组分别上调了44.14%(40.45%)、38.55%(95.17%)、70.65%(83.25%);胚胎期垂体tsh-β基因表达水平在50、500μg/L浓度组显著上调,而幼鱼期tsh-β基因表达水平仅在50μg/L暴露组显著上调。[结论]双酚AF胁迫能够降低斑马鱼胚胎72 h孵化率,crh基因对BPAF胁迫较敏感,最低有影响浓度LOEC为5μg/L。
[Abstract]:[objective] to study the effect of bisphenol A F stress on the early development of zebrafish. [methods] the fertilized eggs were exposed to 50 渭 g / L and 500 渭 g / L of fertilized eggs for 7 days by semi-static exposure. The hatching rate and the expression of related genes were detected by microscopic observation and real-time fluorescence quantitative PCR. [results] 50500 渭 g / L BPAF exposure affected the hatching rate of zebrafish embryos at 72 h, and decreased 44.97% and 49.44%, respectively, while the hypothalamic trh gene did not change significantly in all exposed groups. However, the hypothalamic crh gene was up-regulated by 44.14% and 40.45% in each exposure group, respectively, and the expression level of Tsh- 尾 gene in embryonic pituitary was significantly increased in 50500 渭 g / L group and only in 50 渭 g / L exposure group, and 70.65% in embryo stage, and only in 50 渭 g / L exposure group in immature fish stage, and the expression level of Tsh- 尾 gene was significantly increased in 50 渭 g / L exposure group in embryo stage. The expression of Tsh- 尾 gene in hypothalamus was significantly up-regulated in 50500 渭 g / L group and only in 50 渭 g / L group. [conclusion] Bisphenol A F stress can reduce the hatching rate of zebrafish embryos at 72 h and CRH gene is sensitive to BPAF stress, and the lowest LOEC concentration is 5 渭 g / L.
【作者单位】：海南医学院热带医学与检验医学院;海南大学海口市环境毒理学重点实验室;海南大学环境与植物保护学院;
【基金】：海南省科协青年科技人才学术创新计划项目(“微塑料及典型塑化剂对斑马鱼内分泌系统的干扰效应”;No.201513) 海南医学院科研培育基金项目(“六氟双酚A对斑马鱼甲状腺激素水平干扰效应研究”;No.HY2014-013)
【分类号】：R114

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