RP-HPLC法测定市售绿茶饮料中茶多酚和生物碱的含量

发布时间：2018-05-08 09:58

  本文选题：绿茶饮料 + 高效液相色谱法　； 参考：《沈阳药科大学学报》2017年04期

【摘要】：目的建立HPLC同时测定市售绿茶饮料中茶多酚和生物碱含量的方法并考察放置时间对其产生的影响。方法样品经SPE小柱萃取后进样分析。色谱柱为Phenomemex Synergi 4u FusionRP ODS(250 mm×4.6 mm,5μm),流动相为乙腈-水,梯度洗脱,流速为1.0 mL·min~(-1),检测波长为210 nm,柱温为25℃。结果在上述色谱条件下,6个待测成分在测定范围内线性关系良好(r"g0.999 1),平均加样回收率为97.3%~99.1%,RSD"f2.9%(n=9);随放置时间的延长,饮料中茶多酚的含量降低,生物碱含量无明显变化。结论随放置时间的延长,饮料中茶多酚的损失率增加,含量减少,其抗氧化活性有所降低。
[Abstract]:Objective to establish a HPLC method for simultaneous determination of tea polyphenols and alkaloids in green tea drinks and to investigate the effect of storage time on the content of tea polyphenols and alkaloids. Methods the samples were extracted by SPE column and analyzed by injection. The chromatographic column was Phenomemex Synergi 4u FusionRP ODS(250 mm 脳 4.6mm ~ 5 渭 m ~ (-1), the mobile phase was acetonitrile-water, gradient elution, the flow rate was 1.0 mL / min ~ (-1), the detection wavelength was 210nm and the column temperature was 25 鈩，

本文编号：1860945