丁香酚胚胎毒性评价:应用胚胎干细胞的实验模型

发布时间：2018-05-16 15:34

本文选题：丁香酚 + 毒性试验　；参考：《中国组织工程研究》2015年19期

【摘要】：背景:丁香酚在医学及食品领域的应用越来越广泛,其药理学作用也不断被研究和发现。但是,对其毒性的研究尚未建立完整的数据库,特别是发育毒性及致畸性的安全性评价亟需完善。目的:建立胚胎干细胞实验模型的基础上,应用胚胎干细胞实验模型初步评价丁香酚的胚胎毒性。方法:体外培养小鼠成纤维细胞3T3和小鼠胚胎干细胞E14TG2a,MTT法检测阳性对照5-氟尿嘧啶、阴性对照青霉素G和受试物丁香酚对3T3细胞和E14TG2a细胞的细胞毒性,计算3种化合物对E14TG2a细胞和3T3细胞的半数生长抑制浓度值;采用悬滴-悬浮-贴壁法体外诱导胚胎干细胞向心肌细胞分化,根据浓度-反应曲线计算3种化合物对E14TG2a细胞的半数分化抑制浓度值。利用胚胎毒性预测模型预测丁香酚的胚胎毒性。结果与结论:丁香酚对3T3细胞和E14TG2a细胞的增殖均有抑制作用,其半数生长抑制浓度值分别为(3.613±0.192)和(1.799±0.131)mg/L。丁香酚对E14TG2a细胞的心肌分化亦有抑制作用,其半数分化抑制浓度值为(3.501±0.158)mg/L。根据胚胎干细胞实验模型预测模型计算得出丁香酚为强胚胎毒性化合物。这为进一步评价丁香酚的安全性提供了研究数据。
[Abstract]:Background: eugenol has been widely used in medicine and food, and its pharmacological effects have been studied and discovered. However, the study of its toxicity has not yet established a complete database, especially the safety evaluation of developmental toxicity and teratogenicity needs to be improved. Aim: to evaluate the embryonic toxicity of eugenol on the basis of embryonic stem cell model. Methods: the cytotoxicity of 3T3 cells and E14TG2a cells to 3T3 cells and E14TG2a cells were detected by MTT assay in vitro cultured mouse fibroblasts and E14 TG2AX positive control, penicillin G negative control and eugenol. The inhibitory concentrations of the three compounds on E14TG2a cells and 3T3 cells were calculated, and the differentiation of embryonic stem cells into cardiomyocytes was induced by suspension adherent method in vitro. Based on the concentration-response curve, the inhibitory concentration of the three compounds on E14TG2a cells was calculated. The embryo toxicity of eugenol was predicted by using embryo toxicity prediction model. Results and conclusion: eugenol could inhibit the proliferation of 3T3 cells and E14TG2a cells. The median inhibitory concentrations of eugenol were 3.613 卤0.1922 and 1.799 卤0.131 mg / L, respectively. Eugenol also inhibited the myocardial differentiation of E14TG2a cells, and its half inhibitory concentration was 3.501 卤0.158 mg / L. Eugenol as a strong embryotoxic compound was calculated according to the model prediction model of embryonic stem cells. This provides data for further evaluation of the safety of eugenol.
【作者单位】：中山大学附属中山医院肿瘤研究所;
【基金】：国家自然科学基金青年基金项目(81202604) 中国博士后科学基金(2014M562244) 广东省自然科学基金(2014A030310013)~~
【分类号】：R114

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