锰对小鼠脑基底核突触体谷氨酸和γ-氨基丁酸释放的影响

发布时间：2018-05-18 17:55

本文选题：氯化锰 + 突触体　；参考：《环境与健康杂志》2015年06期

【摘要】：目的探讨锰对小鼠基底核突触体谷氨酸(Glu)和γ-氨基丁酸(GABA)释放的影响。方法将24只健康清洁级昆明小鼠按体重随机分为4组,分别为对照(生理盐水)组和25、50、100μmol/kg氯化锰染毒组,每组6只,雌雄各半。采用腹腔注射方式进行染毒,每周染毒5次,连续染毒4周。测量小鼠基底核内的锰含量;并用15 mmol/L KCl诱发并测定基底核突触体Glu和GABA的释放量。结果与对照组比较,50、100μmol/kg氯化锰染毒组小鼠基底核锰含量均增加,差异有统计学意义(P0.05)。且随着锰染毒剂量的升高,小鼠基底核锰含量呈上升趋势。与对照组比较,25、50μmol/kg氯化锰染毒组小鼠基底核突触体Glu的释放量均增加,100μmol/kg氯化锰染毒组小鼠基底核突触体Glu的释放量及50、100μmol/kg氯化锰染毒组小鼠基底核突触体GABA的释放量均下降,差异有统计学意义(P0.05);且随着氯化锰染毒剂量的升高,小鼠基底核突触体Glu释放量呈先上升后下降的趋势,而GABA释放量呈下降的趋势。结论锰可在小鼠脑基底核内蓄积,并对基底核突触体谷氨酸和GABA释放产生影响,造成神经系统紊乱。
[Abstract]:Objective to investigate the effects of manganese on the release of glutamate and GABA in the synaptosomes of the basal nucleus of mice. Methods 24 healthy and clean grade Kunming mice were randomly divided into 4 groups according to their body weight. They were divided into control group (normal saline) group and 25550100 渭 mol/kg manganese chloride group with 6 mice in each group, half male and half female. Intraperitoneal injection was performed 5 times a week for 4 weeks. The content of manganese in the basal nucleus of mice was measured, and the release of Glu and GABA from the synaptosome of the basal nucleus was induced and measured by 15 mmol/L KCl. Results compared with the control group, the content of manganese in basal nucleus of mice exposed to 50100 渭 mol/kg manganese chloride increased, and the difference was statistically significant (P 0.05). The content of manganese in the basal nucleus of mice increased with the increase of the dose of manganese. Compared with the control group, the release of Glu from basal nucleus synaptosomes of mice exposed to manganese chloride at 25 渭 mol/kg increased, and that of Glu from basal nucleus of mice exposed to manganese chloride of 100 渭 mol/kg decreased, and that of GABA from basal nucleus of mice exposed to manganese chloride of 50100 渭 mol/kg decreased. The difference was statistically significant (P 0.05), and with the increase of the dose of manganese chloride, the release of Glu from the synaptosomes of the basal nuclei of mice increased first and then decreased, while the release of GABA decreased. Conclusion Manganese can accumulate in the basal nucleus of the rat and influence the release of glutamate and GABA from the synaptosome of the basal nucleus, resulting in nervous system disturbance.
【作者单位】：中国医科大学公共卫生学院环境卫生学教研室;
【基金】：国家自然科学基金(81372942) 辽宁省高等学校杰出青年学者成长计划(LJQ2014089)
【分类号】：R114

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