燕窝对肺阴虚小鼠免疫功能影响的研究

发布时间：2018-05-19 07:41

本文选题：燕窝 + 肺阴虚小鼠　；参考：《福建农林大学》2017年硕士论文

【摘要】：燕窝是几种金丝燕在繁殖的季节用唾液或唾液与其绒羽混合凝结成的巢窝。燕窝多产自印度尼西亚、泰国、我国西(南)部等东南亚国家以及北太平洋的澳大利亚等国。李时珍的《本草纲目》描述了燕窝入肺生气、入肾滋水、入胃补脾、补而不燥的功能。燕窝因其含有丰富的营养物质,药用价值极高,在全球范围内都深受欢迎。目前,对燕窝功能的研究很多,但燕窝对特定受损器官的调养与恢复影响的相关研究较少,燕窝对肺阴虚动物免疫功能影响的研究未见报道。本课题研究了燕窝对肺阴虚小鼠免疫功能的影响及机理,这将为证实燕窝具有滋养肺阴的功能,并应用于未来开发燕窝调养与阴虚症相关的诸多肺损伤疾病的产品,提供理论依据,研究结果如下:1.燕窝营养价值高,对其氨基酸、无机元素、唾液酸、蛋白质、脂肪、总糖和灰分含量的测定结果为:燕窝匀浆物的总氨基酸含量占49.49%(含9种必需/半必需氨基酸),蛋白质含量占59.53%,唾液酸含量占9.47%,脂肪、总糖和灰分分别占0.04%、0.08%和2.39%,无机元素种类多样,其中宏量元素Ca、Mg、Na和K含量丰富,且含多种人体所需的微量元素。2.利用S02熏蒸法构建肺阴虚动物模型,研究不同浓度燕窝匀浆物对肺阴虚小鼠体征、体重、免疫器官指数、肺部病理变化及血清中细胞因子IL-2、IL-4和IFN-γ含量的影响。结果表明,燕窝中、高剂量匀浆物对提高肺阴虚小鼠的机体免疫功能有一定的作用。燕窝中、高剂量组小鼠和正常对照组一样,没有出现模型对照组小鼠呛咳、倦伏、皮毛光泽差、凌乱脱落、烦躁易怒、食少、体重显著下降等肺阴虚症状;燕窝中、高剂量组和阳性对照组小鼠的免疫器官指数与正常对照组相近,但显著高于模型对照组(P0.05),说明燕窝中、高剂量组小鼠肺部炎症较模型对照组轻微;小鼠肺部HE染色结果显示,燕窝中、高剂量组小鼠肺部的炎症情况介于正常小鼠及模型小鼠之间;燕窝中、高剂量组的IL-2、IFN-γ水平趋近于正常对照组,而显著低于模型对照组(P0.05),各组实验小鼠间IL-4水平无显著差异,提示燕窝对肺阴虚小鼠的恢复可能有一定作用,且燕窝中剂量组的效果较好。3.研究燕窝中剂量对小鼠脾淋巴细胞增殖,脾淋巴细胞凋亡,肺组织中IL-8、丙二醛(MDA)水平的影响及小鼠肺组织基因的变化。燕窝中剂量组的脾淋巴细胞增殖效果显著高于正常对照组(P0.05),而显著低于模型对照组(P0.05),说明燕窝匀浆物能有效减缓肺阴虚小鼠的免疫应激反应。对于小鼠脾淋巴细胞凋亡总比例和早期凋亡比例,模型对照组均显著高于正常对照组(P0.05),燕窝中剂量组早期凋亡细胞比例显著低于模型对照组(P0.05),说明燕窝匀浆物对抑制脾淋巴细胞凋亡具有一定的作用。模型对照组小鼠肺组织的IL-8、MDA比其他组小鼠的水平都高,燕窝中剂量组小鼠肺组织中IL-8、MDA含量均接近正常对照组,提示燕窝匀浆物可能具有抑制机体产生IL-8、MDA的作用,从而抑制炎症反应的发生,减轻小鼠肺组织的损伤。与正常对照组相比,其他三组小鼠肺组织的共同差异基因有三个,分别为:Atrophin-1,Cytokeratin-5 和 Cyclooxygenase-2,燕窝可通过抑制Atrophin-1,Cyclooxygenase-2两种基因表达的上调来延缓肺阴虚小鼠肺组织的损伤,从而增强机体的免疫作用。
[Abstract]:The bird's nest is a nest of saliva and saliva mixed with its feathers in the breeding season. The bird's nest is produced from Indonesia, Thailand, western (South) and other Southeast Asian countries as well as Australia and other countries in the North Pacific. Li Shizhen's < Compendium of Materia Medica > describes the bird's nest into the lungs, enters the kidney and nourish the spleen, and does not fill the spleen. Because of its rich nutrients and high medicinal value, the bird's nest is very popular in the world. At present, many studies have been made on the function of bird's nest, but few studies have been made on the effect of bird's nest on the regulation and recovery of specific damaged organs. The study of the effect of bird's nest on the immune function of lung yin asthenia animals has not been reported. The effect and mechanism of the bird's nest on the immune function of the lung yin deficiency mice will be used to confirm the function of the bird's nest to nourish the lung yin, and to provide the theoretical basis for the future development of the products related to the deficiency of the bird's nest and the deficiency of the Yin. The results are as follows: 1. the nutritive value of the bird's nest is high, and its amino acids, inorganic elements, sialic acid, and eggs are of high value. The results of the determination of white matter, fat, total sugar and ash content were as follows: the total amino acid content of the bird's nest homogenate was 49.49% (including 9 essential / semi essential amino acids), protein content was 59.53%, the content of sialic acid accounted for 9.47%, fat, total sugar and ash accounted for 0.04%, 0.08% and 2.39%, and the inorganic elements were diverse, including the macroelements Ca, Mg, Na and K. The effects of different concentrations of bird's nest homogenate on the physical signs, body weight, immune organ index, lung pathological changes and the content of cytokines IL-2, IL-4 and IFN- in the serum of lung yin asthenia mice were studied by S02 fumigation. The results showed that the high dose homogenate in the bird's nest was extracted by.2.. In the bird's nest, the high dose group, the high dose group and the normal control group, did not appear in the model control group, with no cough, tiredness, poor lustre, disorder and shedding, irritability, less food and a significant decrease in weight, and the immunity of the high dose group and the positive control group in the bird's nest. The organ index was similar to that of the normal control group, but it was significantly higher than the model control group (P0.05). It showed that the lung inflammation in the high dose group was slightly higher in the high dose group than the model control group. The lung HE staining results showed that the lung inflammation in the high dose group of mice was between the normal mice and the model mice; in the bird's nest, the high dose group was IL-2, I The level of FN- gamma is close to the normal control group, but it is significantly lower than the model control group (P0.05). There is no significant difference in the level of IL-4 between the mice in each group. It suggests that the bird's nest may have a certain effect on the recovery of the lung yin deficiency mice, and the effect of the dose group in the bird's nest is better.3. to study the proliferation of splenic lymphocyte in the mouse's nest, the apoptosis of the spleen lymphocyte and the lung. The effect of IL-8, malondialdehyde (MDA) and gene changes in lung tissue in mice. The proliferation effect of spleen lymphocyte in the middle dose group of the bird's nest was significantly higher than that of the normal control group (P0.05), but significantly lower than the model control group (P0.05), indicating that the swallow nest homogenate could effectively alleviate the immune stress response of mice with lung yin deficiency. The proportion of apoptosis and the proportion of early apoptosis in the model control group were significantly higher than that in the normal control group (P0.05). The percentage of early apoptotic cells in the middle dose group of the bird's nest was significantly lower than that of the model control group (P0.05), indicating that the swallow nest homogenate had a certain effect on the inhibition of the apoptosis of the spleen lymphocyte. The IL-8 and MDA of the lung tissue in the model control group were more than that of the other groups. The levels of IL-8 and MDA in the lung tissue of the middle dose group of the bird's nest were all close to the normal control group, suggesting that the swallow nest homogenate may inhibit the production of IL-8 and MDA in the body, thus inhibiting the occurrence of inflammation and reducing the damage of lung tissue in mice. Compared with the normal control group, the common differential genes in the lung tissues of the other three groups of mice were compared. There are three: Atrophin-1, Cytokeratin-5 and Cyclooxygenase-2. The bird's nest can postpone the injury of lung tissue in mice with lung yin deficiency by inhibiting the up regulation of the expression of Atrophin-1 and Cyclooxygenase-2, thus enhancing the immune function of the body.
【学位授予单位】：福建农林大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R151

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