T-2毒素对凡纳滨对虾的经口急性毒性效应研究

发布时间：2018-05-21 01:42

本文选题：T-毒素 + 凡纳滨对虾　；参考：《现代食品科技》2015年01期

【摘要】：通过T-2毒素微胶囊毒饵料一次性染毒凡纳滨对虾,采用模型拟合法测定T-2毒素对凡纳滨对虾经口LD50,并分析Ca2+-ATPase、多酚氧化酶(PPO)活力以及肌肉病理组织学变化,进而探明T-2毒素对凡纳滨对虾急性毒性效应。结果表明,T-2毒素对凡纳滨对虾的急性毒性经口一次性暴露的LD50为1.22 mg/(kg·bw),T-2毒素对PPO酶活性和Ca2+-ATPase活性的急性毒性效应ED50分别为0.05和3.22 mg/(kg·bw),比较风险评估指数RI可知,PPO活性可作为生物学效应标记描述T-2毒素对对虾的急性毒性效应,且比Ca2+-ATPase更加灵敏。T-2毒素急性暴露可导致肌纤维间隙面积比增大,可导致对虾肌肉品质劣化。采用LC-MS/MS定量检测染毒对虾肌肉中的T-2毒素,但是没有发现游离态T-2毒素残留,说明对虾中T-2毒素急性暴露不会引起物质蓄积,但却产生功能蓄积,可能是T-2毒素以隐蔽态形式存在,导致初始轻微损伤逐渐累加的结果。
[Abstract]:The oral LD50 of T-2 toxin against Penaeus vannamei was measured by model fitting method, and the activity of Ca2-AT Pase, polyphenol oxidase (PPO) and the changes of muscle histopathology were analyzed by using T-2 toxin microencapsulated food as a single dose of Penaeus vannamei (Penaeus vannamei). The acute toxicity of T-2 toxin to Penaeus vannamei was investigated. The results showed that the acute toxicity of T-2 toxin to Penaeus vannamei was 1.22 mg/(kg BWW-2 toxin, and the acute toxicity effect of T-2 toxin to PPO activity and Ca2 -ATPase activity was 0. 05 and 3. 22 mg/(kg BwN respectively. Sex can be used as a biological effect marker to describe the acute toxic effect of T-2 toxin on prawns. Moreover, acute exposure of toxin. T-2 was more sensitive than Ca2-ATPase, which resulted in the increase of gap area ratio of muscle fiber and the deterioration of muscle quality of Penaeus penaeus. LC-MS/MS was used to quantitatively detect the T-2 toxin in the muscle of Penaeus penaeus, but no free T-2 toxin residue was found, which indicated that the acute exposure of T-2 toxin in the shrimp did not cause accumulation of substance, but produced the accumulation of function. It is possible that the T-2 toxin exists in the form of concealment, resulting in the gradual accumulation of initial slight damage.
【作者单位】：广东省水产品加工与安全重点实验室广东普通高等学校水产品深加工重点实验室广东海洋大学食品科技学院;
【基金】：国家自然科学基金资助项目(31171634,31371777,31201309,31371746)
【分类号】：R114

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