亚砷酸钠对大鼠学习记忆影响的实验研究

发布时间：2018-05-22 07:51

本文选题：亚砷酸钠 + 学习记忆　；参考：《现代预防医学》2015年01期

【摘要】：目的通过动物实验观察亚砷酸钠对大鼠学习记忆的影响。方法选用40只SD大鼠,随机分为4组并给予不同亚砷酸钠染毒剂量:高剂量组(1.5 mg/(kg·w))、中剂量组(1.0 mg/(kg·w))、低剂量组(0.5 mg/(kg·w))以及空白对照组(0 mg/(kg·w))。利用水迷宫实验及跳台实验观察比较饮用亚砷酸钠大鼠与对照组大鼠神学习记忆的差异,同时采用酶联免疫吸附试验(ELISA)、单细胞凝胶电泳(SCGE彗星实验)等方法对染毒大鼠脑组织中多巴胺(DA)、5-羟色胺(5-HT)含量、单胺氧化酶(MAO)活性及DNA损伤情况进行检测。结果水迷宫实验结果显示,饮用高砷水组与对照组大鼠相比抵达平台游泳时间较长,其差异具有统计学意义(P0.05);跳台实验结果显示,饮用高砷水组的第一次错误次数(EN1)、第二次错误次数(EN2)均高于对照组,且差异有统计学意义(P0.05);饮用高砷水大鼠脑组织中DA含量及MAO酶活力较空白组均有明显下降,并且差异有统计学意义(P0.05);单细胞凝胶电泳实验结果显示,饮用高砷水可引起大鼠脑组织细胞DNA的损伤(P0.05)。结论高砷暴露可影响中枢神经系统功能,具体表现为学习记忆能力下降,其影响机制可能与DNA损伤有关。
[Abstract]:Objective to observe the effect of sodium arsenite on learning and memory in rats. Methods Forty Sprague-Dawley rats were randomly divided into 4 groups and given different doses of sodium arsenite: high dose group (1.5 mg/(kg), middle dose group (1.0 mg/(kg), low dose group (0.5 mg/(kg) and blank control group (0 mg/(kg). The differences of learning and memory between rats drinking sodium arsenite and rats in control group were observed by water maze test and platform jumping experiment. At the same time, the contents of dopamine dadaminerine 5-HT, monoamine oxidase (MAO) activity and DNA damage in the brain tissue of the exposed rats were detected by Elisa and single cell gel electrophoresis (SCGE comet assay). Results the results of water maze test showed that the swimming time to platform was longer in high arsenic drinking water group than that in control group, and the difference was statistically significant (P 0.05). The first error times and the second error times of drinking as water group were higher than those of the control group, and the difference was statistically significant (P 0.05), and the DA content and MAO enzyme activity in brain tissue of rats drinking as high arsenic water were significantly lower than those in the control group, and the contents of DA and the activity of MAO enzyme in the brain tissue of the rats with high arsenic drinking water were significantly lower than those of the control group. The results of single cell gel electrophoresis showed that drinking high arsenic water could induce DNA damage in rat brain tissue cells. Conclusion High arsenic exposure may affect the function of central nervous system, which is manifested by the decrease of learning and memory ability, and its mechanism may be related to DNA damage.
【作者单位】：新疆医科大学公共卫生学院劳动卫生与环境卫生学教研室;
【基金】：国家自然基金项目(81260410)
【分类号】：R114

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