辣椒素对体内胆固醇代谢机理的研究

发布时间：2018-05-27 08:07

  本文选题：辣椒素 + 血脂　； 参考：《西南大学》2012年硕士论文

【摘要】：本文以辣椒素为原料,通过化学分析、仪器分析和动物模型建立,对辣椒素对大鼠体内胆固醇代谢机理进行了研究。为辣椒素在食品加工和饮食健康方面的应用提供了理论依据。其研究主要结论如下： (1)采用32只SD大鼠,雌雄各半,适应性饲养一周后随机分为四组,空白组、低剂量组、中剂量组、高剂量组,每天分别灌胃0、2.5、5.0、7.5mg/kg辣椒素,基础饲料饲养28天。经检测发现辣椒素能降低大鼠的体重,对饲料效率没有显著性影响。辣椒素能降低大鼠血浆和肝脏中胆固醇含量,这种效果存在性别差异,雌性大鼠对辣椒素功效的敏感性高于雄性。辣椒素能降低大鼠肝脏重量、肝脏脂肪含量、肝脏中总胆固醇和甘油三酯总含量。高剂量辣椒素使肝脏内TRPV1的mRNA表达量增加,使CYP7A1、FXR和HMG-CoA的mRNA表达量降低(P0.05),提高大鼠回肠中ASBT和IBABP mRNA的表达量(P0.05)。本实验证明每天灌胃高剂量组辣椒素对刺激大鼠食欲,降低大鼠血脂和肝脂,影响胆固醇代谢循环有较好效果。辣椒素通过降低肝脏胆固醇代谢基因和升高回肠胆汁酸代谢基因,降低体内血脂、肝脂,从而调节胆固醇在体内的代谢循环。 (2)模拟更年期妇女建立动物模型。采用30只雌性SD大鼠,分组进行双侧卵巢切除(OVX)手术和伪切除手术, OVX组大鼠随机分成4组,空白组、低剂量组、中剂量组、高剂量组,每天分别灌胃0、5.0、10.0、15.0mg/kg辣椒素,喂食相同基础饲料28天。经检测发现,OVX空白组大鼠的体重、采食量、血脂、肝脏总脂肪、总胆固醇、甘油三酯总含量均显著高于伪切除组,说明造模成功。辣椒素可降低OVX组大鼠的体重增加量、进食量,对饲料效率没有影响；辣椒素能降低血浆和肝脏中胆固醇浓度；降低大鼠肝脏中脂肪、总TC和总TG含量。辣椒素能升高肝脏中TRPV1的mRNA表达(P0.05),降低肝脏中CYP7A1、FXR和HMG-CoA的mRNA表达(P0.05)；辣椒素能提高大鼠回肠中TRPV1、ASBT和IBABP mRNA的表达(P0.05)。本实验证明,辣椒素对因雌性激素缺乏引起高脂血症雌性大鼠具有较好的降血脂、降肝脂和调节胆固醇代谢的效果,能有效预防雌性大鼠因雌激素缺乏引起的高脂血症的发生。辣椒素通过降低高脂血症大鼠肝脏内胆固醇代谢基因,升高回肠胆汁酸代谢基因,降低体内血脂、肝脂,从而调节胆固醇在体内的代谢循环。 (3)采用48只SD大鼠,雌雄各半,随机分成六组,分别喂食基础、高脂和高胆固醇饲料,每种饲料组又分为灌胃辣椒素组(10mg/kg/d)和不灌胃辣椒素组。 与基础饲料组相比,高脂饲料组大鼠血脂和肝脂显著升高(P0.05),肝脏重量也有升高趋势。肝脏中TRPV1、HMG-CoA的mRNA表达量显著降低(P0.05)；CYP7A1和FXR的mRNA表达量显著升高(P0.05),雌性大鼠肝脏中FXR的mRNA表达量没有显著变化,但有升高趋势。高脂饲料组大鼠回肠中的TRPV1的mRNA显著升高(P0.05),ASBT和IBABP的mRNA显著降低(P0.05)。辣椒素可显著降低高脂饲料组大鼠的血脂和肝脂,升高雄性大鼠肝脏中TRPV1的mRNA的表达量(P0.05),降低FXR和CYP7A1的mRNA的表达量(P0.05),对HMG-CoA的mRNA没有显著性影响,但是有降低趋势。显著升高雌性大鼠肝脏中TRPV1的表达量(P0.05),降低FXR、HMG-CoA和CYP7A1的mRNA表达量(P0.05)。升高大鼠回肠中TRPV1、ASBT、IBABP的mRNA的表达量。 与基础饲料组相比,高胆固醇饲料组大鼠血脂和肝脂升高,肝脏重量也有升高趋势。肝脏中的TRPV1和HMG-CoA的mRNA的表达量显著降低(P0.05),FXR和CYP7A1的mRNA的表达量显著升高(P0.05),回肠中ASBT和IBABP的mRNA的表达量显著降低(P0.05),雌性大鼠回肠中的TRPV1的mRNA显著升高(P0.05)；雄性大鼠回肠中TRPV1的mRNA没有显著变化。辣椒素可降低高胆固醇饲料组大鼠的血脂和肝脂,升高肝脏中TRPV1的mRNA的表达量(P0.05),显著降低FXR、HMG-CoA和CYP7A1的mRNA的表达量(P0.05)；显著升高高胆固醇饲料组大鼠回肠中TRPV1、ASBT和IBABP的mRNA表达量(P0.05)。 本实验证明,辣椒素能有效降低喂食高脂饲料和高胆固醇饲料大鼠的血脂和肝脂,并能有效调节体内胆固醇代谢循环。可见,辣椒素能有效预防高脂饲料和高胆固醇饲料引起的高脂血症。辣椒素通过降低高脂血症大鼠肝脏内胆固醇代谢基因和升高回肠胆汁酸代谢基因,降低体内血脂、肝脂,从而调节胆固醇在体内的代谢循环。
[Abstract]:With capsaicin as raw material, the mechanism of capsaicin's cholesterol metabolism in rats was studied by chemical analysis, instrumental analysis and animal model. The theoretical basis for the application of capsaicin in food processing and diet health was provided.
(1) 32 SD rats and male and female rats were divided into four groups after one week of adaptation. The blank group, the low dose group, the middle dose group and the high dose group were fed with capsaicin 0,2.5,5.0,7.5mg/kg and the basal diet for 28 days. The capsaicin could reduce the weight of the rats and had no significant effect on the feed efficiency. Capsaicin could decrease. The cholesterol content in the plasma and liver of the rats had a gender difference. The sensitivity of the female rats to capsaicin was higher than that in the male. Capsaicin could reduce the liver weight, liver fat content, total cholesterol and triglyceride content in the liver. High dose capsicum increased the mRNA expression of TRPV1 in the liver, and made CYP7A1, The mRNA expression of FXR and HMG-CoA decreased (P0.05) and increased the expression of ASBT and IBABP mRNA in the ileum of rats (P0.05). This experiment proved that capsaicin has a good effect on stimulating rats' appetite, lowering blood lipid and liver fat, and affecting the cholesterol metabolism cycle. Capsaicin can reduce the cholesterol metabolism gene and increase in liver. Ileal bile acid metabolism genes reduce blood lipids and liver fat, thereby regulating the metabolic cycle of cholesterol in the body.
(2) to simulate the animal model of the menopause women. 30 female SD rats were used to perform bilateral ovariectomy (OVX) surgery and pseudo excision. Group OVX rats were randomly divided into 4 groups, blank group, low dose group, medium dose group, high dose group, respectively, respectively, and fed with 0,5.0,10.0,15.0mg/kg capsaicin every day, feeding the same basic feed for 28 days. The test was tested. The total content of weight, feed intake, blood lipid, total fat, total cholesterol and triglyceride in the OVX blank group were significantly higher than that in the pseudo excision group, indicating that the model was successful. Capsaicin could reduce the weight gain of rats in group OVX, and had no effect on the feed efficiency; capsaicin could reduce the concentration of cholesterol in the plasma and liver; reduce the large amount of cholesterol in the liver. Capsaicin can increase the mRNA expression of TRPV1 in the liver (P0.05), and reduce the mRNA expression of CYP7A1, FXR and HMG-CoA in the liver (P0.05). Capsaicin can improve the expression of TRPV1, ASBT, and HMG-CoA in the ileum of rats. It is proved that capsaicin can cause hyperlipidemia female with estrogen deficiency. The effect of reducing blood lipid, reducing liver fat and regulating cholesterol metabolism can effectively prevent the occurrence of hyperlipidemia caused by estrogen deficiency in female rats. Capsaicin can reduce the metabolism of bile acids in the ileum by lowering the cholesterol metabolism gene in the liver of hyperlipidemia rats, reduce the blood lipid and liver fat, and regulate the cholesterol. The metabolic cycle in the body.
(3) 48 SD rats and male and female rats were divided into six groups randomly. They were fed on the base, high fat and high cholesterol diet, and each feed group was divided into capsaicin group (10mg/kg/d) and no capsaicin group.
Compared with the basic diet group, the lipid and liver fat in the high fat diet group increased significantly (P0.05), the liver weight also increased. The mRNA expression of TRPV1 and HMG-CoA in the liver was significantly decreased (P0.05), the mRNA expression in CYP7A1 and FXR increased significantly (P0.05), but the mRNA expression of FXR in the liver of the female rats was not significantly changed, but there was a rising trend. The mRNA of TRPV1 in the ileum of the high fat diet group increased significantly (P0.05), and the mRNA of ASBT and IBABP decreased significantly (P0.05). Capsaicin significantly reduced the blood lipid and liver fat of the rats in the high fat diet group and increased the mRNA expression of TRPV1 in the liver of the male rats (P0.05), and decreased the expression of FXR and CYP7A1. The expression of TRPV1 (P0.05) in the liver of female rats and the mRNA expression of FXR, HMG-CoA and CYP7A1 (P0.05) were significantly increased in the female rat liver. The mRNA expression of TRPV1, ASBT, IBABP in the ileum was increased.
In the high cholesterol diet group, the blood lipid and liver fat increased and the liver weight also increased. The expression of TRPV1 and HMG-CoA in the liver decreased significantly (P0.05), the expression of mRNA in FXR and CYP7A1 increased significantly (P0.05), the mRNA expression of ASBT and IBABP in the ileum was significantly reduced (P0.05), female rats returned. The mRNA of TRPV1 in the intestine increased significantly (P0.05), and the mRNA of the TRPV1 in the ileum of the male rats was not significantly changed. The capsaicin reduced the lipid and liver fat of the high cholesterol diet group, increased the mRNA expression of the TRPV1 in the liver (P0.05), significantly reduced the expression of FXR, HMG-CoA and CYP7A1 mRNA, and significantly increased the high cholesterol feed. The expression of TRPV1, ASBT and IBABP mRNA in the ileum of rats (P0.05).
Capsaicin can effectively reduce blood lipid and liver lipids in high fat feed and high cholesterol diet rats, and can effectively regulate cholesterol metabolism in the body. Capsaicin can effectively prevent hyperlipidemia caused by high fat diet and high cholesterol diet. Capsaicin can reduce the cholesterol metabolism in the liver of rats with hyperlipidemia. Genes and increased ileal bile acid metabolism genes reduce body fat and liver fat, thereby regulating the metabolic cycle of cholesterol in the body.
【学位授予单位】：西南大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R151

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