长期低剂量锰染毒对子代大鼠生精细胞细胞周期阻滞和凋亡的影响

发布时间：2018-05-30 03:44

本文选题：锰 + 细胞周期　；参考：《实用医学杂志》2017年21期

【摘要】：目的探讨氯化锰长期低剂量染毒对子代大鼠生精细胞细胞周期及细胞凋亡的影响。方法健康雌性SD大鼠32只随机分为对照组和锰染毒组,每组8只。锰染毒组腹腔注射2、4和8 mg/kgMnCl_2·4H_2O,对照组腹腔注射等容生理盐水,1次/d,5次/周,共8周。与正常雄性SD大鼠交配受孕后,雌鼠在妊娠期和哺乳期继续染毒。每组随机取8只12周龄子代雄性大鼠断头处死后取材,HE染色观察睾丸生精小管结构;Western Blot检测FOXO3A、P16、P21、CDK2、CDK4、CDK6、BIM和Caspase-9的表达;TU-NEL法检测生精细胞凋亡。结果随锰染毒剂量的增加,生精小管呈现生精细胞层数减少、生精细胞排列紊乱、数量减少等变化;随锰染毒剂量的增加,睾丸组织FOXO3A的表达逐渐上升;P16、P21表达量逐渐增加;CDK2、CDK4则逐渐降低,CDK6的表达未见明显改变;BIM、Caspase-9表达及生精细胞凋亡指数均随锰染毒剂量的增加逐渐增加。结论长期低剂量锰染毒可能通过诱导FOXO3A表达,引起子代大鼠生精细胞的细胞周期阻滞和细胞凋亡。
[Abstract]:Objective to investigate the effects of long term low dose manganese chloride exposure on spermatogenic cell cycle and apoptosis in rat offspring. Methods 32 healthy female Sprague-Dawley rats were randomly divided into control group (n = 8) and manganese exposed group (n = 8). The manganese exposed group was intraperitoneally injected with 2O4 and 8 mg/kgMnCl_2 4H _ 2O, while the control group was intraperitoneally injected with isovolumic physiological saline for 5 times a week for 8 weeks. After mating with normal male SD rats, female rats continued to be poisoned during pregnancy and lactation. In each group, 8 12-week-old male rats were killed at random, and then HE staining was used to observe the structure of seminiferous tubules in testis. Western Blot was used to detect the expression of CDK4 and CDK6BIM and Caspase-9 in testicular seminiferous tubules. Apoptosis of spermatogenic cells was detected by TU-NEL. Results the number of spermatogenic cells in seminiferous tubules decreased with the increase of manganese exposure dose, and the number of spermatogenic cells in spermatogenic tubules decreased with the increase of manganese exposure dose, and the number of spermatogenic cells decreased with the increase of manganese exposure dose. The expression of FOXO3A increased gradually and the expression of P16 + P21 increased gradually. The expression of CDK2 + CDK4 decreased gradually. The expression of Caspase-9 and apoptosis index of spermatogenic cells increased with the increase of the dose of manganese in testicular tissues, but the expression of Caspase-9 and the apoptosis index of spermatogenic cells increased with the increase of manganese exposure dose. Conclusion Long-term low dose manganese exposure may induce cell cycle arrest and apoptosis in spermatogenic cells of offspring rats by inducing FOXO3A expression.
【作者单位】：遵义医学院细胞生物学教研室;湖南省娄底市中心医院生殖医学中心;
【基金】：贵州省自然科学基金(黔科合J字[2011]2282)
【分类号】：R114

【相似文献】