塑化剂邻苯二甲酸二丁酯与小牛胸腺DNA的沟槽结合

发布时间：2018-05-30 12:52

本文选题：邻苯二甲酸二丁酯 + 小牛胸腺DNA　；参考：《分析科学学报》2016年05期

【摘要】：运用多种光谱学方法,结合化学计量学多元曲线分辨-交替最小二乘法(MCRALS),以及分子模拟技术,在人体生理酸度(pH=7.4)条件下研究了邻苯二甲酸二丁酯(DBP)与小牛胸腺DNA(ctDNA)的相互作用。采用MCR-ALS法分析了DBP与ctDNA作用的紫外光谱数据矩阵,结果表明DBP与ctDNA作用形成了DBP-ctDNA复合物。荧光猝灭实验显示,ctDNA对DBP的荧光猝灭属于形成复合物的单一静态猝灭,其主要驱动力为疏水作用和氢键。通过单双链、熔点和粘度等实验证明DBP与ctDNA发生了沟槽结合,结合区域为A、T碱基富集区。圆二色谱和琼脂糖凝胶电泳分析表明,DBP与ctDNA结合导致ctDNA结构变得更为松散,但未造成质粒DNA损伤。分子模拟形象直观地展现了两者的结合姿态,预测结果与实验结果吻合。
[Abstract]:The interaction between dibutyl phthalate (DBP) and calf thymus DNA (ctDNA) was studied under the condition of physiological acidity (pH = 7.4) by means of multiple spectroscopic methods combined with chemometrics multivariate curve resolution and alternating least square method (MCRALSU) and molecular simulation technique under the condition of physiological acidity of human body (pH = 7.4). The interaction between dibutyl phthalate (DBP) and calf thymus DNA (ctDNA) was studied. The UV spectrum data matrix of the interaction between DBP and ctDNA was analyzed by MCR-ALS method. The results showed that the interaction of DBP and ctDNA formed DBP-ctDNA complex. Fluorescence quenching experiments show that the fluorescence quenching of DBP by ctDNA is a single static quenching of the complex, the main driving forces of which are hydrophobic interaction and hydrogen bond. The experiments of single and double chains, melting point and viscosity show that DBP and ctDNA have trench binding, and the binding region is Ampere base rich region. Circular dichroism and agarose gel electrophoresis analysis showed that the binding of ctDNA with ctDNA resulted in looser structure of ctDNA, but no damage to plasmid DNA. The image of molecular simulation shows the combined attitude of the two directly, and the predicted results are in agreement with the experimental results.
【作者单位】：南昌大学食品科学与技术国家重点实验室;
【分类号】：O657.3

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