麦芽酚铝对大鼠肾上腺嗜铬细胞瘤细胞β-淀粉样蛋白42蛋白表达的影响及其作用机制

发布时间：2018-05-30 13:23

本文选题：铝 + PC细胞　；参考：《环境与健康杂志》2017年08期

【摘要】：目的探讨铝对大鼠肾上腺嗜铬细胞瘤(PC12)细胞β-淀粉样蛋白(Aβ)42表达的影响及其作用机制。方法将处于对数生长期的PC12细胞分别暴露于含麦芽酚铝终浓度为0(对照)、50、100、200和400μmol/L的完全培养基染毒12、24、48 h。采用CCK-8法测定细胞活性,采用酶联免疫吸附(ELISA)测定法测定PS1、Aβ42蛋白的含量。结果与对照组比较,200、400μmol/L麦芽酚铝染毒12 h时及各浓度麦芽酚铝染毒24 h、48 h时PC12细胞的存活率均较低,差异有统计学意义(P0.05);且随着麦芽酚铝染毒浓度的升高,不同染毒时间PC12细胞的存活率均呈逐渐降低的趋势。与对照组比较,400μmol/L麦芽酚铝染毒12 h时及200、400μmol/L麦芽酚铝染毒24 h、48 h时PC12细胞PS1蛋白的含量均增加,差异有统计学意义(P0.05)。与对照组比较,400μmol/L麦芽酚铝染毒12 h时及200、400μmol/L麦芽酚铝染毒24 h时以及100、200、400μmol/L麦芽酚铝染毒48 h时PC12细胞Aβ42蛋白的含量均增加,差异有统计学意义(P0.05)。结论γ-分泌酶表达增加可能是铝致PC12细胞Aβ42蛋白含量增多的重要原因之一。
[Abstract]:Objective to investigate the effect of aluminum on the expression of 尾 -amyloid protein A 尾 -chl42 in rat adrenal pheochromocytoma (PC12) cells and its mechanism. Methods PC12 cells at logarithmic growth stage were exposed to a complete medium containing aluminum-maltol at 0 (control group 50100200 and 400 渭 mol/L) for 48 h. The cell activity was determined by CCK-8 and the protein content of PS1 A 尾 42 was determined by Elisa. Results compared with the control group, the survival rate of PC12 cells was lower at 12 h and 24 h and 48 h, respectively, and the difference was statistically significant (P 0.05), and with the increase of maltol aluminum concentration, the survival rate of PC12 cells was significantly lower than that of control group (P < 0.05), and with the increase of maltol aluminum concentration, the survival rate of PC12 cells was significantly lower than that of control group (P < 0.05). The survival rate of PC12 cells decreased gradually at different exposure time. Compared with the control group, the content of PS1 protein in PC12 cells increased at 12 h and 200400 渭 mol/L, 24 h and 48 h, respectively, and the difference was statistically significant (P 0.05). Compared with the control group, the A 尾 42 protein content of PC12 cells increased at 12 h, 200400 渭 mol/L, 24 h and 48 h, respectively, and the difference was statistically significant (P 0.05). Conclusion the increased expression of 纬 -secretase may be one of the important reasons for the increase of A 尾 42 protein content in PC12 cells induced by aluminum.
【作者单位】：山西医科大学公共卫生学院环境卫生学教研室;山西医科大学公共卫生学院劳动卫生学教研室;
【基金】：国家自然科学基金(30972512) 山西省自然科学基金(2013011052-1) 山西医科大学博士启动基金(B03201209)
【分类号】：R114

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