石榴叶多酚对小鼠急性酒精性肝损伤的保护作用

发布时间：2018-06-01 04:58

本文选题：石榴叶多酚 + 急性酒精性肝损伤　；参考：《食品科学》2015年21期

【摘要】：目的:探究石榴叶多酚对酒精所致小鼠急性肝损伤的保护作用。方法:将60只小鼠随机分为空白组,模型组,石榴叶多酚2低、中、高剂量组(100、200、400 mg/(kg·d))和阳性对照组(联苯双酯滴丸,150 mg/(kg·d)),每组10只。空白组与模型组小鼠灌胃去离子水(0.1 m L/(10 g·d)),连续灌胃30 d;第31天,除空白组外,其余组小鼠均灌胃50%乙醇(12 m L/kg)建立急性肝损伤模型,12 h后,取小鼠眼球血清测定生化指标,取肝脏观察肝组织的超微结构。结果:模型组小鼠肝脏天冬氨酸氨基转移酶(aspartate aminotransferase,AST)、丙氨酸氨基转移酶(alanine aminotransferase,ALT)活力及血清中甘油三酯(triglyceride,TG)水平、ALT和AST活力明显升高,亚显微观察显示小鼠肝细胞膜结构异常,线粒体畸形,内质网明显扩张,内质网膜上附着的核糖体脱落,肝细胞内出现较多脂滴;随着给药剂量的增加,石榴叶多酚2各剂量组对上述情况的改善作用也不断加强。结论:石榴叶多酚2对小鼠急性酒精性肝损伤具有一定的保护作用。
[Abstract]:Objective: to investigate the protective effect of pomegranate leaf polyphenols on acute liver injury induced by alcohol in mice. Methods: sixty mice were randomly divided into blank group, model group, pomegranate leaf polyphenol 2 low, medium and high dose group (100 200400 mg/(kg / d) and positive control group (biphenyl diester drop pill 150 mg/(kg / d) with 10 rats in each group. Mice in the blank group and the model group were perfused with deionized water (0.1 mL / L / 10 g / d) for 30 days, and on the 31st day, the mice in the other groups were given 50% ethanol (12 mL / kg) to establish the model of acute liver injury for 12 h, and the eyeball serum was taken from the mice to determine the biochemical indexes. The liver was taken to observe the ultrastructure of liver tissue. Results: the activity of aspartate aminotransferase (AST), alanine aminotransferase (alt) and triglyceride-triglyceride (TGG) in serum were significantly increased in the model group, and the ultrastructure of liver cell membrane was abnormal by submicroscopic observation. Mitochondria deformity, endoplasmic reticulum dilatation, ribosome attached to endoplasmic omentum and lipid droplets appeared in hepatocytes. With the increase of dosage, the effect of pomegranate leaf polyphenol 2 on the above situation was strengthened. Conclusion: pomegranate leaf polyphenol 2 has protective effect on acute alcoholic liver injury in mice.
【作者单位】：南京师范大学金陵女子学院;
【基金】：江苏省普通高校研究生科研创新计划项目(KYLX_0729)
【分类号】：R151

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