氟染毒对大鼠皮肤成纤维细胞中SOST及DKK1蛋白表达的影响

发布时间：2018-06-02 20:44

本文选题：硬骨素 + DKK　；参考：《环境与职业医学》2017年10期

【摘要】：[目的]观察氟染毒对体外培养新生大鼠皮肤成纤维细胞硬骨素(sclerostin,SOST)和Dickkopf-1(DKK1)蛋白表达的影响。[方法]采用原代培养方法,将大鼠皮肤成纤维细胞分为对照组(0 mg/L)和7个染氟剂量组(0.000 1、0.001、0.01、0.1、1、10、20 mg/L),分别在4个染氟时间段(24、48、72、96 h)收集细胞培养上清液,应用ELISA法测定SOST和DKK1蛋白表达水平。[结果]与染氟0 mg/L组相比,SOST表达量在染氟48 h的0.000 1、0.001、0.01 mg/L组,染氟72 h的1、10 mg/L组及染氟96 h的0.001、0.01、0.1、1 mg/L组均明显下降(P0.05)。与染氟24 h组相比,SOST表达量在染氟48 h的0.01 mg/L组,染氟96 h的0.001、0.01、0.1、1、10 mg/L组均明显下降(P0.05)。与染氟0 mg/L组相比,DKK1表达量在染氟48 h的0.001 mg/L组,染氟72 h的0.01、0.1、1、10、20 mg/L组及染氟96 h的0.01、0.1、1、10、20 mg/L组均明显下降(P0.05)。与染氟24 h组相比,DKK1表达量在染氟48 h的0.000 1、0.001、0.01 mg/L组,染氟72 h的0.001、0.01、1、10、20 mg/L及染氟96 h的0.01、0.1、1、20 mg/L组均明显下降(P0.05)。[结论]氟可导致大鼠皮肤成纤维细胞SOST和DKK1蛋白表达下调。
[Abstract]:[objective] to observe the effect of fluoride exposure on the expression of osteostatin (sclerostinine) and Dickkopf-1 (DKK1) protein in cultured skin fibroblasts of neonatal rats in vitro. [methods] Rat skin fibroblasts were divided into two groups: control group (0 mg / L) and fluorine-exposed group (0.000 1g / L). The supernatants were collected from rat skin fibroblasts at 4 fluorosis time periods (24 ~ 448 ~ 7296 h). The expression of SOST and DKK1 protein was determined by ELISA assay. [results] compared with the fluorine 0 mg/L group, the expression of sst was significantly decreased in the 0.000 1U 0.001 mg/L, 72 h 10 mg/L and 96 h fluorinated 0. 01 mg/L groups, respectively (P 0. 05, P 0. 05, P 0. 05, P 0. 05, P 0. 05, P 0. 05, P 0. 05, P 0. 05, P 0. 05, P 0. 05, P 0. 05, P 0. 05, P 0. 05). Compared with the 24 h fluoride exposure group, the expression of sst was significantly decreased in the 0. 01 mg/L group at 48 h, and the 0. 001 0. 01 + 0. 1 + 1 + 10 mg/L group at 96 h after fluoride exposure (P 0. 05, P 0. 05, P 0. 05, P 0. 05, P 0. 05). Compared with the 0 mg/L group, the expression of DKK1 in the 0.001 mg/L group at 48 h, the 0. 01 ~ 0. 1 + 10 ~ 10 ~ 20 mg/L group in 72 h fluoride exposure group and the 0. 01 0. 1 1 + 10 ~ 10 ~ 20 mg/L group in 96 h fluoride group were significantly decreased (P 0. 05). Compared with the 24 h group, the expression of DKK1 was significantly decreased in the 0.000 1U 0. 001 mg/L group at 48 h, the 0. 001 0. 01 0. 01 mg/L in 72 h fluoride exposure group, and the 0. 01 + 0. 1 0. 01 mg/L group in 96 h fluoride exposure group. The expression of DKK1 protein was significantly decreased in the 72 h fluorine exposure group (P 0. 05) compared with the 24 h fluorine exposure group (P 0. 05). [conclusion] fluoride can induce down-regulation of SOST and DKK1 protein expression in rat skin fibroblasts.
【作者单位】：山东省淄博市疾病预防控制中心;北京大学医学部公共卫生学院流行病与卫生统计学系;华中科技大学同济医学院公共卫生学院劳动卫生与环境卫生学系;
【基金】：国家自然科学基金项目(编号:81072255、81372941)
【分类号】：R114

【相似文献】