神经病靶酯酶在三邻甲苯基磷酸酯抑制小鼠精子发生的作用

发布时间：2018-06-05 04:15

本文选题：三邻甲苯磷酸酯 + 神经病靶酯酶　；参考：《南昌大学》2013年硕士论文

【摘要】：目的：三邻甲苯磷酸酯（TOCP）常作为增塑剂、软化剂及阻燃剂等，在工业中使用非常广泛，本课题主要观察TOCP对小鼠睾丸曲细精管及精子发生的影响，，探究神经病靶酯酶（NTE）在TOCP抑制小鼠精子发生中的作用。方法：1、用HE染色法观察TOCP对小鼠睾丸曲细精管及精子发生的影响；2、用免疫组织化学方法检测NTE在小鼠睾丸中表达；3、用戊酸苯酯水解法检测TOCP对小鼠睾丸NTE活性的影响4、用RT-PCR法检测TOCP对小鼠睾丸组织中NTE mRNA表达的影响。结果：1、分别用不同浓度（0,100,200,400mg/kg）的TOCP给成年雄性小鼠连续染毒14d后，结果显示，与对照组相比，200和400mg/kg TOCP处理组小鼠的睾丸系数明显降低（P＜0.05），而100mg/kg浓度的TOCP则并没有明显抑制小鼠的睾丸系数（P0.05）。不同浓度的TOCP对附睾系数则无明显差异（P0.05）。2、与对照组相比，200和400mg/kg TOCP处理组小鼠睾丸的曲细精管结构显著紊乱，其生精细胞脱落，而100mg/kg TOCP处理组小鼠的睾丸曲细精管则无明显破坏。与对照组相比，100，200及400mg/kg的TOCP均可抑制精子的数量（P＜0.05）。3、免疫组织化学结果显示，除曲细精管的表层细胞之外，NTE在曲细精管的不同生精细胞（包括精原干细胞、初级精母细胞、次级精母细胞和精子）均高表达。4、TOCP呈浓度依赖式地抑制小鼠睾丸中的NTE活性（P＜0.05），但对小鼠睾丸中的NTE mRNA表达无明显的影响（P0.05）。结论：1、TOCP降低睾丸系数而对附睾系数无明显影响；2、TOCP明显损伤睾丸的曲细精管并抑制精子发生；3、NTE在小鼠睾丸组织中表达；4、TOCP抑制睾丸中NTE活性而对其mRNA表达无明显影响。
[Abstract]:Objective: Trio-toluene phosphate (TOCP) is widely used in industry as plasticizer, softener and flame retardant. The effect of TOCP on testicular seminiferous tubules and spermatogenesis in mice was observed. To explore the role of neuropathic target esterase (NTE2) in the inhibition of spermatogenesis in mice by TOCP. Methods: 1. The effect of TOCP on testicular seminiferous tubules and spermatogenesis in mice was observed by HE staining, and NTE was detected by immunohistochemistry in testis of mice. The effect of TOCP on the activity of NTE in testis of mice was detected by phenyl valerate hydrolysis. (4) the effects of TOCP on the expression of NTE mRNA in testis of mice were detected by RT-PCR. The results showed that compared with the control group, the testicular coefficient of mice treated with TOC 200 and 400mg/kg was significantly lower than that of the control group (P < 0.05), while the TOC with 100mg/kg concentration did not significantly inhibit the testicular coefficient of mice (P 0.05). Compared with the control group, the testicular seminiferous tubules of the mice treated with different concentrations of TOCP were significantly disordered and spermatogenic cells were shedded, but the testicular seminiferous tubules of the mice treated with 100mg/kg TOCP had no obvious damage to the seminiferous tubules. Compared with the control group, TOC of TOC 100 200 and 400mg/kg could inhibit the number of spermatozoa (P < 0 05. 3). The immunohistochemical results showed that NTE was present in different spermatogenic cells (including spermatogonial stem cells and primary spermatocytes) in the seminiferous tubules except the surface cells of the seminiferous tubules. Both secondary spermatocytes and spermatozoa) were highly expressed. (4) TOCP inhibited NTE activity in mouse testis in a concentration-dependent manner (P < 0.05), but had no significant effect on NTE mRNA expression in testis of mice (P < 0.05). Conclusion: TOC can decrease testicular coefficient but not epididymis coefficient. TOC significantly affected the damage of testicular tubules and inhibited spermatogenesis in testicular tissues of mice. TOC inhibited the activity of NTE in testis, but had no effect on its mRNA expression.
【学位授予单位】：南昌大学
【学位级别】：硕士
【学位授予年份】：2013
【分类号】：R114

【参考文献】