纳米二氧化硅颗粒对肝HL-7702细胞的毒性作用

发布时间：2018-06-06 11:28

本文选题：纳米SiO颗粒 + HL-细胞　；参考：《吉林大学学报(医学版)》2015年05期

【摘要】：目的:探讨纳米二氧化硅(SiO2)颗粒对肝细胞HL-7702的毒性作用,阐明其作用机制。方法:体外培养的人正常HL-7702肝细胞随机分为阴性对照组和12.5、25.0、50.0、100.0mg·L-1 SiO2组。采用透射电子显微镜(TEM)和动态光散射(DLS)法对纳米SiO2颗粒进行表征和粒径检测。细胞处理24h后,HE染色观察各组细胞形态学;MTT实验检测各组细胞存活率;乳酸脱氢酶(LDH)活力实验检测各组细胞培养液中LDH活力;流式细胞术(FCM)检测各组细胞中活性氧(ROS)水平。结果:TEM观察,纳米SiO2呈球形,颗粒大小较均匀一致,分散性较好,颗粒平均粒径为(65.87±9.02)nm;DLS法检测,纳米SiO2颗粒在高纯水和RPMI-1640培养液中的水合粒径明显增大,分别为(124.57±8.02)和(139.32±9.93)nm。HE染色,纳米SiO2颗粒能够导致HL-7702细胞形态改变,25.0和50.0 mg·L-1 SiO2组细胞数目减少,排列紊乱;100.0mg·L-1 SiO2组细胞质皱缩、细胞排列稀疏,部分细胞呈凋亡的形态学表现。与阴性对照组比较,各浓度纳米SiO2组HL-7702细胞存活率均下降,50.0和100.00mg·L-1 SiO2组细胞存活率明显下降(P0.05);50.0和100.00mg·L-1 SiO2组培养液中LDH活力明显升高(P0.05),细胞中ROS水平明显升高(P0.05)。结论:纳米SiO2颗粒能够对HL-7702细胞产生毒性作用,并呈一定的浓度依赖效应;其毒性作用机制可能与细胞中ROS的产生有关联。
[Abstract]:Aim: to investigate the toxic effect of nano-silica sio _ 2 particles on HL-7702 in hepatocytes and to elucidate its mechanism. Methods: cultured human normal HL-7702 hepatocytes were randomly divided into two groups: negative control group and 12.525. 0 渭 g / L SiO2 group. Transmission electron microscopy (TEM) and dynamic light scattering (DLS) were used to characterize and measure the particle size of SiO2 nanoparticles. After 24 hours of cell treatment, the cell viability was detected by HE staining, the activity of lactate dehydrogenase (LDH) was measured by MTT assay, the activity of LDH in culture medium was detected by flow cytometry (FCM), the level of reactive oxygen species (Ros) in the cells was detected by flow cytometry (FCM). Results SiO2 nanoparticles were spherical in size, uniform in size and good in dispersity. The average particle size was 65.87 卤9.02 nmdls. The hydration diameter of SiO2 nanoparticles in high purity water and RPMI-1640 medium was obviously increased, which was 124.57 卤8.02 and 139.32 卤9.93)nm.HE, respectively. SiO2 nanoparticles could decrease the number of HL-7702 cells in 25.0 and 50.0 mg L ~ (-1) SiO2 groups, while the cells in the disorder 100.0mg L ~ (-1) SiO2 group were reduced in cytoplasm, cell lines were sparse, and some of the cells showed apoptosis. Compared with the negative control group, the survival rate of HL-7702 cells in different concentrations of nano SiO2 group was significantly decreased. The survival rate of HL-7702 cells in 100.00mg L-1 SiO2 group and 100.00mg L-1 SiO2 group was significantly decreased. The activity of LDH in the culture medium of P0.05 and 100.00mg L-1 SiO2 groups was significantly increased, and the ROS level in the cells was significantly increased in the P0.05G group compared with the negative control group. Conclusion: SiO2 nanoparticles can produce toxic effect on HL-7702 cells in a dose-dependent manner, and the mechanism of its toxicity may be related to the production of ROS in the cells.
【作者单位】：吉林大学公共卫生学院卫生毒理学教研室;西安医学院公共卫生系;首都医科大学公共卫生学院劳动卫生与环境卫生学系;首都医科大学公共卫生学院卫生化学与毒理学系;
【基金】：国家自然科学基金资助课题(81172704)
【分类号】：R114

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