高浓度氢气对四氯化碳损伤性大鼠睾丸生精细胞Akt表达的影响

发布时间：2018-06-06 10:38

本文选题：氢气 + 四氯化碳　；参考：《右江民族医学院学报》2016年06期

【摘要】：目的研究吸入高浓度氢气后对四氯化碳损伤性大鼠生精细胞内Akt表达的影响。方法选取健康雄性3月龄SD大鼠30只,随机平均分为对照组(Con组),四氯化碳组(CCl_4组)和氢气组(H_2组)。Con组大鼠于每周一、周五皮下注射橄榄油(0.07ml/100g),CCl_4组和H_2组于每周一、周五皮下注射30%CCl_4+70%橄榄油(0.1ml/100g),持续6周;H_2组在第6周加入高浓度H_2(3%H_2+97%空气)治疗,每天1h,连续7d。取睾丸,应用免疫组织化学和Western blot方法检测Akt的表达水平。结果免疫组化结果显示,Akt主要表达于精原细胞和初级精母细胞的胞浆中,CCl_4组大鼠生精细胞内Akt表达水平较对照组明显降低,H_2组较CCl_4组表达水平升高(P0.01)。Western blot结果显示与免疫组化结果相一致。结论高浓度H_2可以增加CCl_4损伤性大鼠睾丸组织中Akt的表达。
[Abstract]:Objective to study the effect of inhaling high concentration of hydrogen on the expression of Akt in the spermatogenic cells of rats with carbon tetrachloride injury. Methods 30 healthy male 3 month old SD rats were randomly divided into control group (group Con), carbon tetrachloride group (group CCl_4) and hydrogen group (H_2 group).Con rats were subcutaneously injected with olive oil (0.07ml/100g) and CCl_4 on Friday (0.07ml/100g). Group and H_2 group were subcutaneously injected with 30%CCl_4+70% olive oil (0.1ml/100g) every Monday and Friday for 6 weeks. Group H_2 was treated with high concentration of H_2 (3%H_2+97% air) at sixth weeks, 1H, 7d. taking every day, immunohistochemistry and Western blot method were used to detect Akt expression. Results immunohistochemistry showed that Akt was mainly expressed in spermatogonia. In the cytoplasm of cells and primary spermatocytes, the expression level of Akt in the spermatogenic cells of group CCl_4 was significantly lower than that in the control group. The expression level of.Western blot in group H_2 was higher than that in CCl_4 group (P0.01), and the results of.Western blot showed the same with the immunohistochemical results. Conclusion high concentration H_2 can increase the expression of Akt in the testicular tissue of rats with CCl_4 injury.
【作者单位】：菏泽医学专科学校解剖学教研室;
【基金】：山东省高等学校科技计划项目(J13LK20)
【分类号】：R114

【相似文献】