反式脂肪酸对小鼠神经行为的影响研究

发布时间：2018-06-07 10:15

本文选题：反式脂肪酸 + 神经行为　；参考：《河北医科大学》2013年硕士论文

【摘要】：目的：建立小鼠运动、学习-记忆神经行为测试组合。应用该组合研究反式脂肪酸对小鼠神经行为功能的影响。为评价反式脂肪酸的健康风险提供科学依据。方法：小鼠适应性7d后，随机分组，设一个对照组和4个反式脂肪酸剂量组，每组24只，雌雄各半。反式脂肪酸在各组的剂量分别为供能比的0.25％、0.5％、1％、2％，连续干预12周，对照组给予普通饲料，每3天记录饲料消费量，每7天记录小鼠体重。12周后测量神经行为指标。选择悬尾实验、强迫游泳实验、旷场实验、Morris水迷宫实验作为神经行为测试组合。数据采用IBM SPSS Statistics统计软件进行数据分析，，应用单因素方差分析和多因素重复测量方差分析。结果：实验过程中动物无死亡，各组小鼠体重在饲养期间与对照组相比无明显变化。旷场实验中各组小鼠中央区域停留时间的差异无统计学意义；强迫游泳实验雌性各组小鼠的静止时间无显著性差异，M5组静止时间明显高于M4组，差别有统计学意义；悬尾实验各组小鼠的静止时间无显著性差异；Morris水迷宫实验各组小鼠逃避潜伏期无显著性差异。结论：根据神经行为测试结果，证实较低剂量的反式脂肪酸（2％以下）不会对小鼠产生明显的行为功能改变。但由于高剂量组雄性小鼠在强迫游泳实验中表现出和对照组的显著差异，因此，反式脂肪酸是否对小鼠的行为产生影响，尚需要进一步的研究。
[Abstract]:Objective : To study the effects of trans fatty acids on neurobehavioral function in mice and to provide scientific basis for evaluating the health risks of trans fatty acids .

Methods : Mice were randomly divided into 4 trans fatty acid groups ( 0.25 % , 0.5 % , 1 % , 2 % ) after 7 days of adaptation . The dosage of trans fatty acids was 0.25 % , 0.5 % , 1 % , 2 % , 12 weeks after continuous intervention .

Results : There was no death in the animals during the experiment , and the body weight of each group was not significantly changed compared with the control group during the feeding period . There was no significant difference in the time of the central area of the mice in the open field experiment .
There was no significant difference in the quiescent time of the female groups in the forced swimming test , and the quiescent time of M5 group was significantly higher than that in M4 group , and the difference was statistically significant .
There was no significant difference in the resting time of the mice in the suspension experiment group .
There was no significant difference in the escape latency of mice in Morris water maze test group .

Conclusion : According to the results of neurobehavioral test , it is shown that the lower dose of trans fatty acid ( below 2 % ) does not produce obvious behavioral changes to the mice . However , since the male mice in the high dose group show significant difference in the forced swimming test and the control group , it is necessary to further study whether the trans fatty acid has an influence on the behavior of the mice .
【学位授予单位】：河北医科大学
【学位级别】：硕士
【学位授予年份】：2013
【分类号】：R151

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