亚砷酸钠暴露对胎鼠肺泡Ⅱ型上皮细胞雌激素受体α表达的差异研究

发布时间：2018-06-07 22:13

本文选题：ERα + AECⅡ　；参考：《现代预防医学》2017年04期

【摘要】：目的研究亚砷酸钠(Na As O2)暴露对雌雄ICR胎鼠鼠肺Ⅱ型上皮细胞(AECⅡ)雌激素受体α(ERα)表达的影响。方法孕期20 d的ICR胎鼠,分辨雌雄,以Ⅰ型胶原酶分离AECⅡ,以免疫黏附法和差速贴壁法纯化细胞,以免疫荧光法鉴定纯度;以0.5、1.25、5μmol/L Na As O2分别处理AECⅡ12 h和24 h;以不含Na As O2的培养基处理AECⅡ12 h和24 h作空白对照组,每组分设6个重复剂量。以实时荧光定量PCR检测各组ACEⅡ内ERαm RNA表达情况,以western blot检测各组ERα蛋白的表达情况。结果免疫荧光显示纯化后AECⅡ纯度为85%±5%;经Na As O2处理12 h,雌性AECⅡ中各剂量组ERαm RNA和中、高剂量组的蛋白表达高于同剂量组雄性(P0.05);雌性各剂量组ERαm RNA和蛋白表达均高于同性别对照组;雄性中剂量组蛋白表达高于雄性对照组。经Na As O2处理24 h,雌性低剂量组ERαm RNA表达和中、高剂量组蛋白表达高于同剂量组雄性(P0.05);雌雄各剂量组ERαm RNA和中高剂量组蛋白表达均高于各自对照组,雌雄对照组之间ERαm RNA和蛋白表达差异无统计学意义(P0.05)。结论亚砷酸钠暴露可致雌性胎鼠AECⅡ内ERα表达高于雄性。
[Abstract]:Objective to study the effect of sodium arsenite (Na As O2) exposure on the expression of estrogen receptor alpha (ER alpha) in the lung type II epithelial cells (AEC II) of rats and male ICR fetal mice. Methods ICR fetal rats with 20 d during pregnancy were identified and identified as AEC II by type I collagenase. The cells were purified by immunofluorescence and differential adherence, and the purity was identified by immunofluorescence, and 0.5,1.25,5 u mo. L/L Na As O2 treated AEC II 12 h and 24 h respectively; AEC II 12 h and 24 were treated as blank control groups without Na As O2, and each group had 6 repeated doses. The purity of II was 85% + 5%; treated with Na As O2, 12 h, and ER alpha m RNA and in each dose group of female AEC II, the protein expression in high dose group was higher than that in the same dose group (P0.05), and the expression of ER alpha m RNA and protein in the female dose group was higher than that in the same sex control group; the middle dose group of the male group was higher than the male control group. The expression of ER alpha m RNA in the dose group and high dose group was higher than that in the same dose group (P0.05), and the expression of ER alpha m RNA and high dose group protein in each dose group was higher than that of the control group. The difference of ER a m RNA and protein expression between the female and male control group was not statistically significant (P0.05). Conclusion the sodium arsenite exposure could cause the female fetal rat AEC II. The expression of alpha is higher than that of male.
【作者单位】：四川大学华西公共卫生学院(华西第四医院);昆明市疾病预防与控制中心;
【基金】：国家自然科学基金资助项目(No.81160340)
【分类号】：R114

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