虾青素对秀丽隐杆线虫衰老的影响及其机制的初步研究

发布时间：2018-06-08 02:27

本文选题：虾青素 + 秀丽隐杆线虫　；参考：《现代食品科技》2015年09期

【摘要】：本文研究了虾青素对秀丽隐杆线虫衰老和正常生理功能的影响及其相关作用机制。试验设立对照组和虾青素组(0.08 m M、0.16 m M、0.40 m M、0.80 m M),观察N2野生型线虫的寿命及相关生理指标(生殖能力、吞咽频率和移动力),评价不同浓度虾青素对线虫衰老过程的影响,明确虾青素作用的最佳剂量;选用最佳剂量的虾青素处理N2野生型、daf-16基因缺陷型和age-1基因缺陷型线虫,分析其寿命长短,探讨虾青素在胰岛素信号通路上延缓线虫衰老的作用机理。结果表明,虾青素可显著延长线虫的寿命(P0.05),0.16 m M虾青素的作用效果最佳,线虫的平均寿命达到26.03 d,显著延长了31.68%;虾青素对线虫的生殖能力和吞咽频率没有显著影响,能显著增强10 d和15 d线虫的移动力。0.16 m M虾青素可显著提高age-1缺陷型线虫的寿命,对daf-16缺陷型线虫的寿命无显著影响。因此,虾青素可显著延长线虫寿命,其作用机制与胰岛素信号通路上的daf-16基因密切相关。
[Abstract]:In this paper, the effects of astaxanthin on senescence and normal physiological function of Cryptophthora elegans and its related mechanism were studied. The control group and astaxanthin group were set up as control group and astaxanthin group. The longevity and physiological indexes (reproductive capacity, swallowing frequency and motility) of N 2 wild type nematodes were observed, and the effects of different concentrations of astaxanthin on the senescence process of nematode were evaluated. The optimal dose of astaxanthin was determined, and the life span of N 2 wild type nematodes (Ndaf-16 and age-1 gene deficient nematodes) was analyzed by using the best dosage of astaxanthin. To study the mechanism of astaxanthin on delaying the senescence of nematode in insulin signaling pathway. The results showed that astaxanthin could significantly extend the life span of Antaxanthin (P 0.05) 0.16m M astaxanthin had the best effect, the average life span of nematode reached 26.03 days, significantly prolonged 31.68 days, and astaxanthin had no significant effect on the reproductive ability and swallowing frequency of nematode. Astaxanthin significantly increased the migration power of 10 d and 15 d. Astaxanthin could significantly increase the longevity of age-1 deficient nematodes, but had no significant effect on the life span of daf-16 deficient nematodes. Therefore, astaxanthin can significantly prolong the longevity of the worm, and its mechanism is closely related to the daf-16 gene in insulin signaling pathway.
【作者单位】：华南农业大学食品学院;
【基金】：国家自然科学基金资助项目(31401481)
【分类号】：R151

【共引文献】