工频磁场对小鼠胚胎成纤维细胞的细胞周期及增殖的后续影响研究

发布时间：2018-06-13 23:02

本文选题：细胞周期 + 细胞增殖　；参考：《浙江大学》2013年硕士论文

【摘要】：背景：社会的现代化以及普遍化使得电力设备成为人们生活不可或缺的部分,却由此带来了人们日常生活和工作环境中极低频电磁场水平的大幅增加,电力设施等产生的多为工频磁场,引发人们对工频电磁场生物效应的关注和探索,正确客观的评价工频磁场的生物学效应成为公共卫生领域亟待解决的科学问题。流行病学研究、动物学实验及细胞学实验表明极低频磁场可能存在健康相关的生物学影响,相比于电磁场暴露的即时效应研究,对于暴露后的时段里电磁场是否会对机体有后续的生物学影响却少有涉及。细胞增殖是细胞生命过程中的重要进程,也是生物学研究中重要的观测指标,细胞周期的分布可以反映细胞对外界刺激的敏感性,本研究拟观察工频磁场暴露的后续时间内细胞增殖和细胞周期的变化情况,以期初步评价不同暴露条件下工频磁场的后续生物学效应,为该领域的研究提供参考。目的：选用细胞增殖和细胞周期为效应指标,初步评价工频磁场暴露后的后续生物学效应。方法：MEF细胞接种后预培养约12h,暴露于50Hz的工频磁场中,暴露条件分别为：暴露强度为0.5mT、2mT,暴露模式为powerline及sine,暴露时间为8h和24h。采用CCK-8试剂盒法和流式细胞术分别检测暴露结束后的0h、12h、24h、36h、48h时的细胞增殖和细胞周期分布情况。各暴露条件均设有一一对照,每个实验重复至少3次。结果：(1)CCK-8试剂盒检测细胞增殖的实验结果：用SPSS16.0软件对实验检测结果进行协方差分析,在本实验条件下,工频磁场暴露后0-48h未对细胞增殖产生影响；(2)采用流式细胞术检测MEF细胞周期的实验结果：50Hz、0.5mT工频磁场powerline模式下辐照24h,发现在辐照结束后的第24h,暴露组细胞出现了细胞周期的G2/M期阻滞；而在sine模式下辐照8h和24h,却发现在暴露结束后的第24h和第48h,暴露组与对照组相比分布在G0/G1期的细胞比例减少；50Hz、2mT工频磁场powerline模式及sine模式暴露24h,发现在辐照结束后的第36h,暴露组细胞出现了细胞周期的S期阻滞。结论：本实验应用细胞周期和细胞增殖两个效应指标评价工频磁场的后续生物学作用,在本实验条件下未观察到工频磁场作用引起明显后续生物学效应。
[Abstract]:Background: with the modernization and generalization of society, electric power equipment has become an indispensable part of people's lives, but it has brought about a significant increase in the level of very low frequency electromagnetic fields in people's daily life and working environment. Most of the electric power facilities are power frequency magnetic field, which has aroused people's attention and exploration to the biological effect of power frequency electromagnetic field. The correct and objective evaluation of the biological effect of power frequency magnetic field has become a scientific problem to be solved urgently in the field of public health. Epidemiological, zoological and cytological studies have shown that very low frequency magnetic fields may have health-related biological effects, compared with the immediate effects of electromagnetic field exposure. There is little concern about whether the electromagnetic field will have subsequent biological effects on the body during the period after exposure. Cell proliferation is an important process in the course of cell life and an important observation index in biological research. The distribution of cell cycle can reflect the sensitivity of cells to external stimuli. The aim of this study was to observe the changes of cell proliferation and cell cycle during the follow-up time of power frequency magnetic field exposure, in order to evaluate the following biological effects of power frequency magnetic field under different exposure conditions, and to provide a reference for the study in this field. Aim: to evaluate the subsequent biological effects of power frequency magnetic field exposure using cell proliferation and cell cycle. Methods the cells were precultured for 12 hours after inoculation and exposed to 50 Hz power frequency magnetic field. The exposure conditions were as follows: the exposure intensity was 0.5mTX 2mT, and the exposure mode was powerline and Sine. the exposure time was 8h and 24h, respectively. CCK-8 kit and flow cytometry were used to detect cell proliferation and cell cycle distribution at 0 h ~ 12 h ~ 24 h ~ 36 h ~ 48 h after exposure. Each exposure condition was controlled one by one, and each experiment was repeated at least three times. Results the cell proliferation was detected by the CCK-8 Kit. SPSS 16.0 software was used to analyze the covariance of the results. Under the experimental conditions, 0-48 h after exposure to the power frequency magnetic field, the cell proliferation was not affected. (2) flow cytometry was used to detect the cell cycle of MEF cells. The results showed that the G _ 2 / M phase arrest of cell cycle appeared in the exposed group at 24 h after irradiation with 0.5 Mt power frequency magnetic field powerline mode. However, the proportion of cells distributed in G _ 0 / G _ 1 phase decreased in the exposed group at 24 h and 48 h after irradiation in sine mode for 8 h and 24 h, respectively. 50 Hz ~ 2mT power frequency magnetic field powerline mode and sine mode were exposed for 24 h. It was found that S phase arrest of cell cycle appeared in the exposed group at 36h after irradiation. Conclusion: the following biological effects of power frequency magnetic field were evaluated by cell cycle and cell proliferation in this experiment.
【学位授予单位】：浙江大学
【学位级别】：硕士
【学位授予年份】：2013
【分类号】：R131

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