植物甾醇酯对高脂膳食大鼠脂代谢影响及其机制研究

发布时间：2018-06-14 12:06

本文选题：植物甾醇酯 + 高脂膳食大鼠　；参考：《中国油脂》2017年06期

【摘要】：为了研究植物甾醇酯(PSE)对高脂膳食大鼠肝脏脂代谢的作用及其机制,将42只雌性SD大鼠随机均分为对照组、模型组和干预组,分别给予基础饲料、高脂饲料和高脂加质量分数2%PSE饲料24周。监测大鼠的体重;检测血清脂蛋白的水平和肝脏脂质水平;检测血清转氨酶(ALT、AST)活性并对肝脏结构进行病理学观察;分别用Real-time PCR和Western Blot的方法评估肝脏LXRα、CYP7A1、ABCA1、HMGCo A、FXR、SREBP-1c、PPARγ、SIRT1 mRNA的表达水平以及PPARγ和SIRT1蛋白的表达水平。结果表明,PSE降低了血清TC和LDL-C以及肝脏TC的水平,增加了血清HDL-C的水平(P0.05),改善了由高脂膳食导致的肝脏组织病理学改变,降低了血清ALT和AST的水平(P0.05)。PSE增加了肝脏SIRT1 mRNA的表达,减少了CYP7A1mRNA和PPARγmRNA以及PPARγ蛋白的表达(P0.05),对LXRα、ABCA1、HMGCo A、FXR和SREBP-1c mRNA的表达以及SIRT1蛋白的表达没有显著作用。
[Abstract]:In order to study the effect and mechanism of phytosterol ester (PSE) on lipid metabolism in liver of rats with high fat diet, 42 female SD rats were randomly divided into control group, model group and intervention group. High fat feed and high fat plus mass fraction 2% PSE forage for24 weeks. The serum lipoprotein level and liver lipid level were measured, the activity of serum aminotransferase (alt) was detected and the liver structure was observed by pathology. Real-time PCR and Western blot were used to evaluate the expression level of PPAR 纬 -SIRT1 mRNA and the expression of PPAR 纬 and SIRT1 protein in liver. The results showed that PSE decreased the levels of serum TC, LDL-C and liver TC, increased the level of HDL-C, improved the histopathological changes of liver caused by high fat diet, and decreased the levels of serum alt and AST. PSE increased the expression of SIRT1 mRNA in liver. The expression of CYP7A1 mRNA, PPAR 纬 mRNA and PPAR 纬 protein was decreased (P 0.05), but the expression of FXR and SREBP-1c mRNA and SIRT1 protein in LXR 伪 ABCA1HMGCo Amo-FXR and SREBP-1c were not significantly affected.
【作者单位】：武汉科技大学公共卫生学院营养与食品卫生系;
【基金】：2013年BASF国际健康研究基金项目(亚洲)
【分类号】：R151

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