四种温石棉致突变性研究

发布时间：2018-06-14 23:38

本文选题：温石棉 + 致突变性　；参考：《毒理学杂志》2015年05期

【摘要】：目的探讨我国4种典型温石棉的致突变作用。方法将4种温石棉受试物配制成3个染毒剂量组(50、100和200μg/ml)分别染毒V79细胞,同时设阴性和阳性对照组。用单细胞凝胶电泳实验检测其所致DNA损伤(每个剂量组染毒时间分别为24、48和72 h);用V79细胞微核试验检测其染色体畸变(每个剂量组染毒时间为24 h)。结果 (1)单细胞凝胶电泳实验:4种温石棉3个染毒剂量组染毒24、48和72 h所致V79细胞DNA迁移距离、OTM、拖尾率均高于阴性对照组(P0.01),染毒48 h均比染毒24 h增加(P0.01),染毒72 h均比染毒48 h下降(P0.01)。(2)V79细胞微核试验:4种温石棉染毒剂量为50μg/ml时,微核率与阴性对照组比较无差异(P0.05),染毒剂量为100和200μg/ml时,微核率均高于阴性对照组(P0.01),并呈剂量-反应关系(P0.01)。结论 4种温石棉单细胞凝胶电泳实验和V79细胞微核试验致突变阳性,产品安全性值得进一步探讨。
[Abstract]:Objective to study the mutagenicity of four typical chrysotile asbestos in China. Methods four chrysotile samples were prepared into three dose groups of 50 100 渭 g / ml and 200 渭 g / ml, respectively, and the negative and positive control groups were set up at the same time. Single cell gel electrophoresis (SCGE) was used to detect the DNA damage (the exposure time of each dose group was 24 ~ 48 and 72 h respectively) and the chromosome aberration was detected by micronucleus test of V79 cells (the exposure time of each dose group was 24 h). Results 1) single cell gel electrophoresis (SCGE) assay showed that the DNA migration distance of V79 cells was significantly higher than that of negative control group (P 0.01) at 48 h and 72 h after exposure to different doses of chrysotile asbestos. The DNA migration distance of V79 cells exposed to chrysotile at 48 h and 72 h was significantly higher than that of control group. When the micronucleus test was 50 渭 g/ml, the micronucleus test time was 50 渭 g/ml, which was less than 48 hours after exposure to chrysotile asbestos. There was no difference in micronucleus rate between the control group and the negative control group. The micronucleus rate was higher than that in the negative control group at the doses of 100 渭 g/ml and 200 渭 g/ml, and was in a dose-response relationship. Conclusion the single cell gel electrophoresis of four chrysotile asbestos and micronucleus test of V79 cells are positive for mutagenesis, and the safety of the products is worthy of further study.
【作者单位】：四川医科大学公共卫生学院;南充市仪陇县人民医院;西南科技大学;绵阳四0四医院;
【基金】：国家自然科学基金(41472046) 四川省科技厅自然科学基金(14JC0126)
【分类号】：R114

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