钼致小鼠亚急性肝脏毒性研究

发布时间：2018-06-16 14:02

本文选题：钼 + 小鼠　；参考：《辽宁医学院》2015年硕士论文

【摘要】：目的探讨钼对小鼠肝脏的亚急性毒性作用。方法7周龄雄性昆明小鼠50只,适应性喂养一周,按体重随机分为五组,分别为对照组(生理盐水)、3.440mg/kg(1/100 LD50)、6.880mg/kg(1/50 LD50)、34.400mg/kg(1/10 LD50)、68.800mg/kg(1/5 LD50)钼酸钠染毒组,每组10只。腹腔注射钼酸钠进行染毒,染毒剂量为0.2ml/10g,每日一次,连续染毒30天。染毒结束后,测定小鼠肝脏湿量和脏器系数;肝脏中钼含量;血清中天门冬氨酸氨基转移酶(AST)、丙氨酸氨基转移酶(ALT)、乳酸脱氢酶(LDH)的含量及肝脏匀浆里的谷胱甘肽过氧化物酶(GSH-PX)、超氧化物歧化酶(SOD)、丙二醛(MDA)的含量;并在光镜下观察小鼠肝脏组织病理学变化。结果1、染毒结束后,各组小鼠体重均增加,34.400mg/kg、68.800mg/kg染毒组小鼠体重同对照组相比差异具有统计学意义。2、与对照组相比,各组小鼠肝脏钼含量升高,且34.400mg/kg,68.800mg/kg组差异具有统计学意义。3、仅68.800 mg/kg钼酸钠染毒组小鼠血清中AST和LDH含量明显高于对照组,且差异有统计学意义。34.400mg/kg和68.800mg/kg钼酸钠染毒组小鼠血清ALT含量和对照组小鼠比较差异具有统计学学意义。4、各组小鼠肝脏匀浆中SOD活力均下降,且6.880mg/kg、34.400mg/kg、68.800mg/kg同对照组相比差异有统计学意义。各小组小鼠肝脏匀浆中的GSH-PX活力也随之降低,34.400mg/kg和68.800mg/kg同对照组相比差异有统计学意义。肝脏匀浆中的MDA含量则随着染毒剂量的增加而增加,同样34.400mg/kg和68.800mg/kg染毒组和对照组相比差异具有统计学意义。5、组织病理观察发现68.800 mg/kg钼酸钠染毒组肝细胞结构不完整,界限不清晰,形态大小不一,肝细胞空泡变性,炎细胞浸润,肝索排列紊乱,点状坏死,中央静脉狭窄。结论钼在小鼠肝脏中有一定的蓄积作用,高剂量的钼对小鼠肝脏具有损伤作用。
[Abstract]:Objective to study the subacute toxicity of molybdenum on mouse liver. Methods Fifty seven week-old male Kunming mice were fed adaptively for one week. They were randomly divided into five groups according to their body weight: the control group (normal saline 3.440 mg / kg / 100 LD50 / kg 1.880 mg / kg 1 / 50 LD50 + 34.400 mg / kg / 10 LD50 68.800 mg / kg / L / 5 LD50) sodium molybdate exposure group (10 rats in each group). Sodium molybdate was injected intraperitoneally at a dose of 0.2 ml / 10 g once a day for 30 days. At the end of the exposure, the wet amount of liver and organ coefficient, the content of molybdenum in liver and the content of molybdenum in liver of mice were measured. The contents of aspartate aminotransferase (AST), alanine aminotransferase (alt), lactate dehydrogenase (LDH) and glutathione peroxidase (GSH-PX), superoxide dismutase (SOD), malondialdehyde (MDA) in liver homogenate; The histopathological changes of mouse liver were observed under light microscope. Results 1. The body weight of each group increased by 34.400 mg 路kg ~ (-1) 路kg ~ (-1) 68.800 mg 路kg ~ (-1) 路kg ~ (-1) compared with that of the control group (P < 0.01). The molybdenum content in liver of each group was higher than that of the control group. There was significant difference in serum AST and LDH levels in mice exposed to sodium molybdate for 68.800 mg/kg, and the difference was significant in the group of 68.800 mg / kg of 34.400 mg / kg of molybdate. The serum alt content of mice exposed to 68.800mg/kg and sodium molybdate was significantly higher than that of control group. The activity of sod in liver homogenate of each group was decreased, and there was a significant difference between 6.880 mg / kg and 34.400 mg 路kg ~ (-1) 路kg ~ (-1) and 68.800 mg / kg compared with the control group. The activity of GSH-PX in liver homogenate of each group mice also decreased 34.400 mg / kg and 68.800mg/kg significantly compared with the control group. The content of malondialdehyde (MDA) in liver homogenate increased with the increase of dose. Similarly, the difference between 34.400mg/kg and 68.800mg/kg groups was statistically significant. Histopathological observation showed that the hepatocyte structure of 68.800 mg/kg sodium molybdate group was incomplete. The boundary is not clear, the shape is different, the hepatocyte vacuole degeneration, the inflammatory cell infiltration, the hepatic cord arrangement disorder, the dot-like necrosis, the central vein narrow. Conclusion Molybdenum has a certain accumulative effect in mouse liver, and high dose of Mo can damage the liver of mice.
【学位授予单位】：辽宁医学院
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R114

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