2013年夏季北京市售整鸡沙门菌分离株耐药性及耐药机制研究

发布时间：2018-06-18 05:41

本文选题：市售整鸡 + 沙门菌　；参考：《卫生研究》2017年04期

【摘要】：目的了解2013年夏季北京市售整鸡样品中分离的166株沙门菌耐药性及耐药机制。方法采用微量肉汤稀释法对166株沙门菌进行9类11种抗生素的耐药性检测,挑选头孢类耐药株进行超广谱β-内酰胺酶(ESBLs)检测,并对环丙沙星和头孢噻肟双耐药株进行耐药机制分析。结果 94.6%(157/166)的沙门菌至少对一种抗生素耐药,多重耐药株占58.4%,对萘啶酸耐药率最高,为92.2%(153/166),其他依次为氨苄西林(48.8%)、氨苄西林-舒巴坦(44.0%)和四环素(44.0%)。共有27种耐药谱,常见的耐药谱为萘啶酸(n=46,29.3%)、萘啶酸-氨苄西林-氨苄西林/舒巴坦(n=28,17.8%)。47株头孢噻肟耐药株中有41株(87.2%)ESBLs阳性,其中有38株同时对环丙沙星耐药。这38株环丙沙星和头孢噻肟双耐药且ESBLs阳性的沙门菌中存在喹诺酮耐药决定区(QRDRs)、质粒介导喹诺酮耐药(PMQR)和ESBLs等耐药机制,37株测试菌株的GyrA的第83位、87位氨基酸和ParC的57位、80位氨基酸存在不同程度突变。38株测试菌株均检测到不同种类和数量的PMQR基因,如qnrB、qnrS、oqxAB和aac(6’)-Ib-cr等,部分基因存在突变。主要存在的β-内酰胺酶类型为CTX-M型(35株)、TEM型(20株)和OXA型(36株),未检测到SHV型和AmpC型。结论北京市售整鸡中沙门菌整体耐药水平较严重,需强化对肉鸡养殖、屠宰、运输、销售等环节中沙门菌耐药性监测,分析耐药机理及传递机制,同时注意临床感染治疗用药监管。
[Abstract]:Objective to investigate the drug resistance and drug resistance mechanism of 166 Salmonella strains isolated from chicken samples sold in Beijing in summer 2013. Methods the drug resistance of 166 Salmonella strains was detected by broth dilution method, and extended spectrum 尾 -lactamase (ESBLs) was detected in the resistant strains of cephalosporins. The resistance mechanism of ciprofloxacin and cefotaxime was analyzed. Results 94.6% of Salmonella were resistant to at least one antibiotic, the multidrug resistant strain was 58.4%, the highest rate of resistance to naphthyronic acid was 92.2% 15.3 / 166%, the others were ampicillin 48.8%, ampicillin and sulbactam 44.0) and tetracycline 44.0%. A total of 27 drug resistance strains were found. The common resistance spectrum was Naphthyronic acid 466 / 29.3. Among the 47 cefotaxime resistant strains, 41 were positive for ESBLs, and 38 were also ciprofloxacin resistant to ciprofloxacin in 41 of the 47 cefotaxime resistant strains. The results were as follows: (1) Naphthalic acid and ampicillin / sulbactam were resistant to ciprofloxacin at the same time, and 41 of the 47 cefotaxime resistant strains were positive for ESBLs, and 38 of them were also resistant to ciprofloxacin. Among the 38 strains with double resistance to ciprofloxacin and cefotaxime and positive for ESBLs, QRDRsN was found in quinolone-resistance determinants, plasmid mediated quinolone resistance (PMQR) and ESBLs, and the 83rd 87th amino acid and 87th amino acid of GyrA in 37 tested strains. The amino acids at the 57th and 80th position of ParC were mutated to different degrees. All the tested strains detected different kinds and numbers of PMQR genes. For example, qnrBU qnrSnsoqxAB and aacan6chon -Ib-cr, some of the genes were mutated. The main types of 尾 -lactamases were CTX-M Tem (20 strains) and OXA type (36 strains). No SHV or AMPC type was detected. Conclusion the overall drug resistance level of salmonella in whole chicken sold in Beijing is serious. It is necessary to strengthen the surveillance of salmonella resistance in broiler breeding, slaughtering, transportation and sale, and analyze the mechanism of drug resistance and transmission mechanism. At the same time, pay attention to clinical infection treatment drug supervision.
【作者单位】：国家食品安全风险评估中心卫生部食品安全风险评估重点实验室;北京市海淀区疾病预防控制中心;
【基金】：国家食品安全风险评估中心高层次人才队伍建设523项目(2017人才-3-11,2017人才-3-4)
【分类号】：R155.5

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