天津市水产品中副溶血性弧菌及其致病性调查

发布时间：2018-06-18 13:04

本文选题：水产品 + 副溶血性弧菌　；参考：《环境与健康杂志》2015年06期

【摘要】：目的了解天津市不同消费场所生食、非生食、熟制水产品副溶血性弧菌污染状况、致病性和耐药性。方法于2013年4—11月在天津市8个区县的农贸市场、超市、餐饮店、烧烤摊采集水产品(生食、非生食和熟制食品),分离检测副溶血性弧菌,并进行毒力基因和耐药检测。结果非生食生鲜水产品、生食水产品和熟制水产品的副溶血性弧菌检出率分别为19%(44/231)、7.3%(29/397)和8.5%(6/71);79株毒力基因阳性菌中3株trh基因阳性,tdh基因均为阴性;79株毒力基因阳性菌对氨苄西林、阿莫西林、环丙沙星、氯霉素、复方新诺明的耐药率分别为94.9%、44.3%、1.3%、1.3%和3.8%。所有毒力基因阳性菌株均对头孢噻肟、头孢西丁、庆大霉素、萘啶酸和四环素敏感。结论本次调查的水产品中副溶血性弧菌污染较为普遍,部分菌株具有致病性和耐药性。
[Abstract]:Objective to investigate the contamination, pathogenicity and drug resistance of Vibrio parahaemolyticus in raw, non-raw and cooked aquatic products in Tianjin. Methods from April to November 2013, aquatic products (raw, non-raw and cooked food) were collected from farmers' markets, supermarkets, restaurants and barbecue stalls in 8 districts of Tianjin. Virulence genes and drug resistance of Vibrio parahaemolyticus were isolated and detected. Results the detection rates of virulent vibrio parahaemolyticus in raw aquatic products, raw aquatic products and cooked aquatic products were 1944 / 231C / 29 / 397, respectively, and 3 of the 79 virulence gene-positive strains with trh gene positive were negative for ampicillin. The resistance rates of amoxicillin ciprofloxacin chloramphenicol and compound sulfamethoxifen were 94. 9% and 3. 8% respectively. All virulence gene-positive strains were sensitive to cefotaxime, cefoxitin, gentamicin, naphthalic acid and tetracycline. Conclusion Vibrio parahaemolyticus contamination is common in aquatic products investigated, and some strains have pathogenicity and drug resistance.
【作者单位】：天津市疾病预防控制中心食品安全科;
【基金】：原天津市卫生局科技基金(2012KY16)
【分类号】：R155.5

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