不同泌乳期人乳磷脂成分的研究

发布时间：2018-06-18 23:15

本文选题：人乳 + 泌乳期　；参考：《上海交通大学学报(医学版)》2017年08期

【摘要】：目的·获得上海地区部分人乳磷脂成分的最新数据,比较不同泌乳期人乳磷脂成分的差异。方法·招募上海交通大学医学院附属新华医院产科2016年4—7月足月分娩的健康产妇。采集第3日的初乳、第10日的过渡乳、第45日的成熟乳,Folch法提取人乳脂肪后经固相萃取分离磷脂,用高效液相色谱-可变波长检测器对磷脂酰乙醇胺、磷脂酰胆碱及鞘磷脂进行定量分析。采用单因素方差分析和Games-Howell检验比较不同泌乳阶段人乳中磷脂成分的差异。结果·入选能提供至少1份母乳的乳母100名,共收集初乳70份、过渡乳96份、成熟乳82份。成熟乳总磷脂含量平均为(281.93±118.54)μg/g,明显低于初乳的(381.99±205.90)μg/g;在泌乳期的各个阶段,磷脂酰胆碱相对含量均最高(53.74%~59.36%),鞘磷脂相对含量其次(28.12%~32.74%);随着泌乳期的延长,磷脂酰乙醇胺相对含量恒定(P=0.617),磷脂酰胆碱相对含量逐渐减少(P=0.000),而鞘磷脂相对含量逐渐增加(P=0.000)。结论·鞘磷脂和磷脂酰胆碱是人乳磷脂的主要成分;人乳磷脂含量随泌乳期延长而改变,成熟乳磷脂总量低于初乳和过渡乳;磷脂酰乙醇胺相对含量在各泌乳阶段保持一致,磷脂酰胆碱相对含量逐渐减少,而鞘磷脂相对含量逐渐增加。
[Abstract]:Objective to obtain the latest data of human lactophospholipid in Shanghai and to compare the differences of human lactophospholipid in different lactation stages. Methods healthy puerperas were recruited from Xinhua Hospital of Shanghai Jiaotong University Medical College to give full term delivery from April to July 2016. The third day of colostrum, the 10th day of transition milk, and the 45 th day of mature milk Folch method were used to extract human milk fat and separate phospholipids by solid phase extraction. Phosphatidyl ethanolamine was determined by high performance liquid chromatography-variable wavelength detector. Phosphatidylcholine and sphingomyelin were quantitatively analyzed. Single factor analysis of variance (ANOVA) and Games-Howell test were used to compare the difference of phospholipid composition in different lactation stages. Results 100 nursing mothers were selected, including 70 colostrum, 96 transitional milk and 82 mature milk. The average content of total phospholipid in mature milk was 281.93 卤118.54 渭 g / g, which was significantly lower than that in colostrum (381.99 卤205.90) 渭 g / g. At all stages of lactation, the relative content of phosphatidylcholine reached the highest level (53.74 卤59.36g), followed by the relative content of sphingomyelin (28.1232.744.74). When the relative content of phosphatidyl ethanolamine was constant, the relative content of phosphatidylcholine decreased gradually, while the relative content of sphingolipid increased. Conclusion sphingomyelin and phosphatidylcholine are the main components of human lactophospholipid, and the content of human lactic phospholipid changes with the prolongation of lactation period, and the total amount of mature lactic phospholipid is lower than that of colostrum and transitional milk. The relative content of phosphatidylcholine decreased gradually, while the relative content of sphingomyelin increased gradually.
【作者单位】：上海交通大学公共卫生学院;
【分类号】：R151.3

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