同位素内标-液质联用法检测猪肉中的苯二氮卓类药物残留

发布时间：2018-06-19 15:34

  本文选题：同位素内标 + 液质联用　； 参考：《现代预防医学》2017年03期

【摘要】：目的建立液质联用同位素内标法同时测定猪肉中硝西泮、氯硝西泮、氟硝西泮、奥沙西泮、劳拉西泮、阿普唑仑、替马西泮、地西泮的方法。方法在均质的猪肉样品中添加同位素内标地西泮-d5,经乙腈提取后用全自动固相萃取仪净化,液相色谱质谱联用仪检测,内标法定量。结果苯二氮卓类镇静剂药物在线性范围内具有良好的线性关系,线性相关性r≥0.999,检出限在0.030μg/kg~0.096μg/kg之间,加标回收率在89.8%-114.6%之间,相对标准偏差在2.45%~7.92%之间。结论该方法灵敏度高、回收率好,适用于猪肉中多种苯二氮卓类镇静剂药物残留的同时检测。
[Abstract]:Objective to establish a method for simultaneous determination of nitrazepam, clonazepam, flunitrazepam, oxazepam, lorazepam, alprazolam, temazepam and diazepam in pork by liquid-mass spectrometry. Methods the isotopic diazepam d5 was added to the homogenized pork samples. After extraction by acetonitrile, it was purified by automatic solid-phase extraction instrument, detected by liquid chromatography-mass spectrometry (LC-MS), and measured by internal standard method. Results the linear range of benzodiazepines was linear, the linear correlation was r 鈮，

本文编号：2040358