苹果渣和迷迭香提取物改善果糖诱导大鼠胰岛素抵抗的作用机制

发布时间：2018-06-24 15:10

本文选题：AR + CD36　；参考：《重庆医科大学》2017年硕士论文

【摘要】：目的苹果渣是苹果加工成果汁、苹果酒或葡萄酒的副产品。迷迭香是一种草药,通常在食品加工中用作香料和调味剂。有证据表明,苹果渣和迷迭香都具有丰富的生物活性分子与众多的代谢作用。为了提供更多关于使用苹果渣和迷迭香作为功能性食品用于改善代谢相关疾病的信息,本研究探讨了苹果渣和迷迭香提取物(a mixture of apple pomace and rosemary extract,AR)改善胰岛素敏感性的作用及机制。方法33只SD雄性大鼠随机分成正常对照组(n=6)与果糖组(n=27)。果糖组给予10%果糖水溶液。于实验第13周,果糖组随机分为果糖对照组,AR低、高剂量(100 mg·kg-1、500 mg·kg-1)组,每组9只,继续果糖水溶液喂养。实验周期为18周,第18周末禁食14小时后,称体质量,大鼠脱颈处死,用ELISA法测定血浆脂质及葡萄糖代谢指标,Real-time PCR检测相关基因表达,Western blot和免疫荧光检测相关蛋白表达水平。结果AR(500mg/kg)治疗5周抑制了长期果糖诱导所致的大鼠空腹血浆胰岛素浓度的升高,降低了胰岛素抵抗指数和脂肪组织胰岛素抵抗指数。从总体而言,AR抑制了口服葡萄糖耐量试验(oral glucose tolerance test,OGTT)期间果糖诱导血浆游离脂肪酸(non-esterified fatty acid,NEFA)清除率的增加,并且降低了腓肠肌中甘油三酯(triglyceride,TG)的浓度和油红-O染色面积。此外,AR逆转了果糖诱导肌膜上脂肪酸转运酶/CD36(fatty acid translocase/CD36,CD36)的过表达,并且上调了肌膜上葡萄糖转运蛋白-4(glucose transporter-4,GLUT-4)的表达,已知CD36促进脂肪酸的摄取,而GLUT-4是胰岛素响应的葡萄糖载体蛋白,它们与胰岛素抵抗发生密切相关。结论AR通过调控肌膜上的CD36和GLUT-4来改善果糖诱导大鼠的胰岛素抵抗。
[Abstract]:Objective Apple pomace is a by-product of apple juice, cider or wine. Rosemary is an herb that is often used as a spice and seasoning in food processing. There is evidence that apple pomace and rosemary are rich in bioactive molecules and many metabolic functions. In order to provide more information on the use of apple pomace and rosemary as functional foods to improve metabolic related diseases, this study investigated the effects and mechanisms of apple pomace and rosemary extract (a mixture of apple pomace and rosemary extract AR on improving insulin sensitivity. Methods 33 Sprague-Dawley male rats were randomly divided into normal control group (n = 6) and fructose group (n = 27). Fructose group was given 10% fructose aqueous solution. At the 13th week of the experiment, fructose group was randomly divided into fructose control group with low AR and high dose (100mg kg-1500 mg kg-1) with 9 rats in each group, fed with fructose aqueous solution. The experimental period was 18 weeks. After fasting for 14 hours at the 18th week, the rats were killed by body weight. The plasma lipid and glucose metabolism index were detected by Elisa. Western blot and immunofluorescence were used to detect the expression of related genes. Results AR (500mg/kg) inhibited the increase of fasting plasma insulin concentration induced by fructose for 5 weeks, and decreased insulin resistance index and adipose tissue insulin resistance index. In general, AR inhibited the increase of free fatty acid (non-esterified fatty acididine) clearance in plasma during oral glucose tolerance test (oral glucose tolerance test), and decreased the concentration of triglyceride (TG) and the area of oil red -O staining in gastrocnemius muscle. In addition, AR reversed the overexpression of fructose induced fatty acid transporter / CD36 (fatty acid translocase / CD36 / CD36 / CD36, and up-regulated the expression of glucose transporter-4 (GLUT-4). It is known that CD36 promotes fatty acid uptake, while GLUT-4 is a glucose carrier protein responsive to insulin. They are closely associated with insulin resistance. Conclusion AR can improve insulin resistance induced by fructose by regulating CD36 and GLUT-4 on myocytes.
【学位授予单位】：重庆医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：TS218;R151

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