新疆库车小白杏杏仁油调节大鼠血脂作用及机制的实验研究

发布时间：2018-06-24 15:21

本文选题：杏仁油 + 营养成分　；参考：《新疆医科大学》2012年硕士论文

【摘要】：目的：以新疆库车小白杏杏仁油为研究对象，对小白杏杏仁油的营养成分进行测定并探讨小白杏杏仁油调节大鼠血脂作用及其机制。方法：依据各营养成分测定国家标准对新疆库车小白杏杏仁油的营养成分进行测定与评价。将50只Wistar雄性大鼠在实验环境下经基础饲料适应性喂养一周，测定大鼠血清总胆固醇（TC），甘油三酯(TG)，高密度脂蛋白胆固醇(HDL-C)和低密度脂蛋白胆固醇（LDL-C）水平。根据TC水平随机将大鼠分为五组：高剂量组、中剂量组、低剂量组、正常对照组和模型组。三个剂量组在给予高脂饲料的同时灌胃不同剂量（低剂量1ml/kg·d，中剂量2ml/kg·d，高剂量4ml/kg·d）的杏仁油，每日一次，正常对照组喂以普通饲料，模型组喂以高脂饲料，对照组和模型组每天给予4ml/kg·d纯净水。八周后抽取大鼠血液离心后测定血清中的总胆固醇（TC），甘油三酯(TG)，高密度脂蛋白胆固醇(HDL-C)和低密度脂蛋白胆固醇（LDL-C）水平；载脂蛋白AI、载脂蛋白B；三碘甲状腺原氨酸(T3)及胰岛素水平；取大鼠肝脏，称重记录并制作组织切片并冻存肝脏用实时荧光定量法测定ApoAI和ApoB基因表达水平。结果：1．每100g新疆库车小白杏杏仁油中含粗脂肪99.5g、碳水化合物0.2g、水分0.1g、杂质0.2g、维生素A0.003mg、ɑ-维生素E0.06mg。2．小白杏杏仁油脂肪酸中含有71%油酸、22.7%亚油酸。3．小白杏杏仁油中含有钙0.663mg/100g、钠0.828mg/100g、钾0.39mg/100g、铁0.083mg/100g、锌0.054mg/100g。4．正常对照组的大鼠肝湿重显著低于其他各组，高剂量组大鼠的肝湿重低于模型组。5．中、高剂量杏仁油组大鼠的血清TC、TG含量不同程度低于模型组，中、高剂量杏仁油组大鼠血清HDL-C含量显著高于模型组。6．各剂量组大鼠的ApoAI含量明显高于模型组；模型组大鼠ApoB含量高于空白组，各剂量组与模型组ApoB含量相比没有差别。7．各剂量组和空白组大鼠体内胰岛素、T3含量显著高于模型组。8.模型组ApoAI基因表达水平低于空白对照组，高剂量组ApoAI基因表达水平高于模型组。模型组ApoB基因表达水平高于空白对照组。结论：新疆库车小白杏杏仁油中碳水化合物和矿物质元素含量丰富，中、高剂量组能降低高血脂大鼠TC、TG水平，提高血清中HDL-C的含量，通过对ApoAI、ApoB、胰岛素和T3的含量的调节，增强ApoAI基因表达，，直接或间接影响血脂水平。
[Abstract]:Objective: to determine the nutritional components of small white apricot almond oil in Xinjiang Kuqa apricot almond oil and to explore the effect and mechanism of small white apricot almond oil on regulating blood lipids in rats. Methods: the nutritional components of apricot oil from Kuqa Xiaobai apricot in Xinjiang were determined and evaluated according to the national standards. Fifty male Wistar rats were fed with basic diet for one week to determine the levels of serum total cholesterol (TC), triglyceride (TG), high density lipoprotein cholesterol (HDL-C) and low density lipoprotein cholesterol (LDL-C). According to TC level, rats were randomly divided into five groups: high dose group, middle dose group, low dose group, normal control group and model group. Almond oil of different doses (low dose 1ml/kg d, middle dose 2ml/kg d, high dose 4ml/kg d) was given to the three dose groups once a day. The normal control group was fed with normal diet and the model group with high fat diet. The control group and the model group were given 4ml/kg d purified water daily. The serum levels of total cholesterol (TC), triglyceride (TG), high density lipoprotein cholesterol (HDL-C) and low density lipoprotein cholesterol (LDL-C) were measured after 8 weeks of blood centrifugation. The levels of triiodothyronine (T3) and insulin were measured by real-time fluorescence quantitative method, and the expression levels of ApoAI and ApoB genes were measured by real-time fluorescence quantitative method. Results: the content of crude fat, carbohydrate, moisture, impurity, vitamin A and vitamin E were 99.5g, 0.2g, 0.2g, 0.003mg, 0.06mg.2.The fatty acids of almond oil contained 71% oleic acid, 22.7wt% linoleic acid .3.The contents of crude fat were 99.5g, 0.2g, 0.2g, 0.2g, 0.003mg, 0.06mg.2.The fatty acids of almond oil contained 71% oleic acid and 22.7wt% linoleic acid. Oil contains calcium 0.663mg / 100g, sodium 0.828mg / 100g, potassium 0.39mg / 100g, iron 0.083mg / 100g, zinc 0.054mg / 100g.4. the wet weight of liver in the normal control group is significantly lower than that in the other groups. The liver wet weight of rats in high dose group was lower than that in model group .5.The content of serum TCN TG in high dose almond oil group was lower than that in model group. The content of serum HDL-C in high dose almond oil group was significantly higher than that in model group. 6. The ApoAI content in each dose group was significantly higher than that in model group, and the content of ApoB in model group was higher than that in blank group. There was no difference in ApoB content between each dose group and model group. The content of insulin T 3 in each dose group and blank group was significantly higher than that in model group. The expression level of ApoAI gene in the model group was lower than that in the blank control group, and the expression level of ApoAI gene in the high dose group was higher than that in the model group. The expression level of ApoB gene in model group was higher than that in blank control group. Conclusion: the content of carbohydrate and mineral elements in the almond oil of Kuqa Xiaobai apricot in Xinjiang is rich. The high dose group can decrease the level of TCU TG and increase the content of HDL-C in serum of rats with hyperlipidemia, and regulate the contents of ApoAIN ApoB, insulin and T3. Enhance the expression of ApoAI gene, directly or indirectly affect the level of blood lipids.
【学位授予单位】：新疆医科大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R151

【参考文献】