食品添加剂纳米二氧化硅对胃肠道细胞的毒性研究

发布时间：2018-06-25 19:57

本文选题：食品添加剂 + 纳米二氧化硅　；参考：《上海大学》2013年硕士论文

【摘要】：二氧化硅(SiO_2)作为一种食品添加剂，已经在食品行业使用了很多年，作为抗结剂防止颗粒聚集结块、保持食品松散或自由流动。随着生产工艺的发展，食品添加剂SiO_2已经达到了纳米尺寸，然而目前关于纳米SiO_2的安全性研究大都从职业暴露角度出发，关于食品添加剂纳米SiO_2的生物性安全数据却很匮乏。考虑到食品添加剂纳米SiO_2会随着食物经口进入到机体内，而胃肠道系统将会是最重要的吸收部位，因此，我们研究了食品添加剂纳米SiO_2对胃肠道细胞的毒性。首先，随机从不同食品添加剂厂商购买了四种不同的食品添加剂二氧化硅(S1，S2，S3，S4)，利用TEM、XRD、FT-IR、DLS、BET等技术对其进行了全面的分析表征，发现四种食品添加剂SiO_2粒径均在10-50nm，在纳米尺寸范围，比表面积较大，表面基团是羟基，，颗粒带负电。在此基础上，本论文研究了这四种食品添加剂纳米SiO_2对人胃粘膜上皮细胞GES-1和人结肠癌细胞Caco-2的毒性影响及作用机制。纳米SiO_2对细胞活力的影响存在剂量和时间效应，样品之间有差异。纳米SiO_2不造成细胞膜损伤、形态改变、细胞的凋亡与坏死，但会抑制细胞的增殖。纳米SiO_2会被胃肠道细胞大量摄入，而且导致轻微的氧化应激反应。纳米SiO_2对两种细胞的毒性影响和机制相似，主要通过阻滞细胞停留在S期来抑制细胞的增殖，但是GES-1细胞比Caco-2细胞要敏感一些。总体来说，低剂量下的食品添加剂纳米SiO_2是比较安全的。本论文还研究了纳米SiO_2在Caco-2细胞单层膜的跨膜情况，发现纳米SiO_2穿膜率很低，说明纳米SiO_2很难穿越小肠膜。此外，本论文还对从口香糖中提取的纳米TiO_2的胃肠道细胞的毒性进行了初步的研究，发现提取出的纳米TiO_2相对比较安全。本论文为纳米食品添加剂的生物安全性评价提供了基础而重要的数据。
[Abstract]:As a food additive, SiO2 has been used in the food industry for many years, as an anti-agglomeration agent to prevent particles from agglomeration and to keep food loose or free flow. With the development of production technology, the food additive SiOSnap2 has reached nanometer size. However, at present, most of the research on the safety of nano-SiOStack2 is based on occupational exposure, but the biological safety data on food additive nano-SiOStack2 is very scarce. Considering that the food additive nano-SiO2 will enter the body with food through the mouth and the gastrointestinal system will be the most important absorption site, we have studied the toxicity of nano-SiO-2 to gastrointestinal cells. First of all, four different kinds of food additive silicon dioxide (S1S2OS3S4) were purchased randomly from different food additive manufacturers. They were characterized by TEMX XRDU FT-IRT-DLSSET and other techniques. It was found that SiO2 of the four kinds of food additives were all in the range of 10-50 nm, in the nanoscale size range. The specific surface area is large, the surface group is hydroxyl, and the particles are negatively charged. On this basis, the toxic effects and mechanism of these four food additives, nano-SiOSTA2, on human gastric mucosal epithelial cells GES-1 and human colon cancer cell line Caco-2 were studied in this paper. The effects of nano-SiO _ 2 on cell viability have dose and time effects, and there are differences among the samples. Nano-SiO _ 2 did not cause cell membrane damage, morphological changes, cell apoptosis and necrosis, but inhibited cell proliferation. Nano-SiO-2 is heavily ingested by gastrointestinal cells and causes a slight oxidative stress response. The toxic effect and mechanism of nano-SiO-2 on the two kinds of cells were similar, mainly by stopping cells in S phase to inhibit cell proliferation, but GES-1 cells were more sensitive than Caco-2 cells. In general, low-dose food additive nano-SiO-2 is relatively safe. In this paper, we also studied the transmembrane of nano-SiOstack _ 2 in Caco-2 cell monolayer. It was found that nano-SiO-2 has a low penetration rate, which indicates that nano-SiO-2 is difficult to penetrate the small intestinal membrane. In addition, the toxicity of nano-TiO-2 extracted from chewing gum was studied, and it was found that the extracted nano-TiO-2 was relatively safe. This paper provides basic and important data for biological safety evaluation of nano-food additives.
【学位授予单位】：上海大学
【学位级别】：硕士
【学位授予年份】：2013
【分类号】：R114;TS202.3

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