2015年洛阳市食品加工从业人员致泻性大肠埃希氏菌带菌状况调查

发布时间：2018-06-25 19:40

本文选题：致泻性大肠埃希氏菌 + 多重PCR　；参考：《现代预防医学》2017年05期

【摘要】：目的建立洛阳市食品加工从业人员携带致泻性大肠埃希氏菌多重PCR分型的基线数据。方法现场采集餐饮服务单位和学校食堂食品加工从业人员的粪便或肛拭子标本150份,通过培养挑取可疑目标菌落,采用多重PCR核酸扩增加毛细管电泳方法进行鉴定、分型与分析。结果 2015年洛阳市共检测食品加工从业人员粪便样本150例,检出致泻性大肠埃希氏菌阳性样本为21例,检出率为14%。其中致病性大肠埃希氏菌(EPEC)检出率最高,检出12例(检出率为8%);其次为粘附性大肠埃希氏菌(EAEC),检出9例(检出率为6%),侵袭性大肠埃希氏菌(EIEC)、产毒性大肠埃希氏菌(ETEC)和肠出血性大肠埃希氏菌(EHEC)均未检出。结论 EAEC和EPEC是该次调查中调查对象携带的主要致泻大肠菌型,应用多重PCR加毛细管电泳技术能快速鉴别粪便标本中的致泻大肠埃希菌。
[Abstract]:Objective to establish the baseline data for multiplex PCR typing of diarrhea Escherichia coli among food processing workers in Luoyang. Methods 150 feces or anal swabs from food processing workers in catering service units and school canteens were collected on the spot and identified by multi-PCR amplification and capillary electrophoresis. Classification and analysis. Results in 2015, 150 cases of food processing workers' feces were detected in Luoyang city, and 21 cases of diarrhea Escherichia coli were detected, and the positive rate was 14.4%. The detection rate of pathogenic Escherichia coli (EPEC) was the highest. 12 cases (8%) were detected, and 9 cases (6%) were found as adherent Escherichia coli (EAEC), but none were found in invasive Escherichia coli (EIEC), toxic Escherichia coli (ETEC) and enterohemorrhagic Escherichia coli (EHEC). Conclusion EAEC and EPEC are the main diarrhea coliforms carried by the subjects in this investigation. Multiple PCR and capillary electrophoresis can be used to identify rapidly the diarrhea Escherichia coli in fecal samples.
【作者单位】：洛阳市疾病预防控制中心检验中心;
【分类号】：R155.7

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