铝对斑马鱼幼鱼的神经毒性作用及其转录组学研究

发布时间：2018-06-26 05:59

本文选题：氯化铝 + 神经毒性　；参考：《广东医学》2017年06期

【摘要】：目的探讨铝对斑马鱼的神经毒性作用及其在转录水平的机制。方法选取受精1 d后发育良好的斑马鱼胚胎,随机分为2组,分别给予单纯培养基(对照组)和50μg/L浓度的AlCl_3处理6 d(AlCl_3处理组)。检测幼鱼自主运动行为、活性氧(ROS)释放及转录组学变化。结果与空白对照组相比,经AlCl_3处理后的斑马鱼,表现出运动轨迹明显无规律,游泳速度减慢,差异有统计学意义(P0.05);AlCl_3增加斑马鱼幼鱼的活性氧释放(P0.05);转录组学数据显示,AlCl_3处理组与对照组相比,Neurogenin 1等19个与退行性神经疾病相关的基因表达发生显著变化(P0.0001),22个显著变化的基因富集在MAPK信号通路。结论铝对斑马鱼具有神经毒性作用,其分子机制可能与Neurogenin 1等19个基因表达的变化及MAPK信号通路的调控有关。
[Abstract]:Objective to investigate the neurotoxicity of aluminum to zebrafish and its mechanism at transcription level. Methods zebrafish embryos were randomly divided into two groups: single medium (control group) and ALCL _ 3 treated with 50 渭 g / L AlCl3 for 6 days (ALCL _ 3 treatment group). The spontaneous exercise behavior, the release of reactive oxygen species (Ros) and transcriptome changes in juvenile were detected. Results compared with the blank control group, the zebrafish treated with AlCl3 showed obvious irregular track and slow swimming speed. The difference was statistically significant (P0.05) that AlCl3 increased the release of reactive oxygen species (P0.05), and transcriptional data showed that the expression of 19 genes related to neurogenin 1 and neurodegenerative neuropathy in the AlCl3 treatment group was significantly different from that in the control group (P0.0001), and 22 were significant. The altered genes are enriched in the MAPK signaling pathway. Conclusion Aluminum has neurotoxic effect on zebrafish and its molecular mechanism may be related to the changes of neurogenin 1 gene expression and the regulation of MAPK signaling pathway.
【作者单位】：遵义医学院珠海校区生物工程系珠海市中药基础及应用研究重点实验室;澳门大学中华医药研究院中药质量研究国家重点实验室;
【基金】：国家自然科学基金资助项目(编号:81328025) 珠海市优势学科建设项目
【分类号】：R114

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