叶黄素对大鼠心肌纤维化保护作用

发布时间：2018-06-29 17:34

本文选题：纤维化 + 叶黄素　；参考：《中国公共卫生》2017年11期

【摘要】：目的探讨叶黄素(lutein)对异丙肾上腺素(ISO)诱导的大鼠心肌纤维化保护作用及机制。方法 60只大鼠随机分为6组,除对照组外,其余各组大鼠皮下注射ISO 5 mg/kg,连续7 d;造模第2 d,低、中、高剂量叶黄素组大鼠灌胃给予lutein 20、40、80 mg/kg,阳性对照组大鼠给予卡托普利(cap)10 mg/kg,模型组和对照组大鼠给予等量蒸馏水,连续28 d。检测大鼠心脏指数、心电图、运动耐力、心肌组织中羟脯氨酸(HYP)、谷胱甘肽(GSH)、丙二醛(MDA)含量和血清中超氧化物歧化酶(SOD)含量;苏木素-伊红染色(HE)观察大鼠心肌病理学变化。结果与对照组比较,模型组大鼠运动耐力降低、心指数与左心室指数[分别为(3.73±0.58)、(2.57±0.43)mg/g]增加(P0.01),心率明显增加,心肌组织中HYP、MDA含量[分别为(0.48±0.07)g/mg、(2.37±0.84)nmol/mgpr]升高(P0.05),心肌组织中GSH含量、血清中SOD水平[分别为(55.19±2.08)U/mg、(512.17±26.48)U/m L]明显下降(P0.01),心肌组织间质成分增多,细胞核间距较宽,有大量炎性细胞浸润且纤维组织增生伴心肌萎缩;与模型组比较,高剂量叶黄素组大鼠运动耐力提高、心指数与左心室指数[分别为(3.02±0.28)、(2.09±0.17)mg/g]降低(P0.01),心肌组织中HYP和丙二醛含量[分别为(0.28±0.06)g/mg、(1.89±0.61)nmol/mgpr]明显降低(P0.05),心肌组织中GSH含量、血清中SOD水平[分别为(72.70±0.05)U/mg、(571.19±15.58)U/m L]明显升高(P0.01),心肌组织间质成分略增多,无炎细胞浸润,可见轻度纤维组织增生,无心肌萎缩。结论叶黄素对异丙肾上腺素所致大鼠心肌纤维化具有一定保护作用,其机制可能与改善大鼠机体抗氧化能力有关。
[Abstract]:Objective to investigate the protective effect and mechanism of lutein (lutein) on isoproterenol induced myocardial fibrosis in rats. Methods Sixty rats were randomly divided into 6 groups, except the control group, rats in other groups were subcutaneously injected with ISO 5 mg / kg for 7 days. Rats in the high dose lutein group were given lutein 20 ~ 400.80 mg / kg by gavage, captopril (cap) was given 10 mg / kg in the positive control group, and the model group and the control group were given the same amount of distilled water for 28 days. Cardiac index, electrocardiogram, exercise endurance, hydroxyproline (HYP), glutathione (GSH), malondialdehyde (MDA) and serum superoxide dismutase (SOD) were measured, and the pathological changes of myocardium were observed by hematoxylin eosin staining (HE). Results compared with the control group, the exercise endurance decreased, the cardiac index and left ventricular index [(3.73 卤0.58), (2.57 卤0.43) mg/g] increased (P0.01), the heart rate increased significantly, and the content of HYPMDA in myocardial tissue [(0.48 卤0.07) g / mg, (2.37 卤0.84) nmol/mgpr] increased (P0.05), respectively. The levels of SOD in serum [(55.19 卤2.08) U / mg, (512.17 卤26.48) U / mL] were significantly decreased (P0.01), the interstitial components of myocardial tissue increased, the nuclear spacing was wider, a large number of inflammatory cells infiltrated, and the fibrous tissue proliferated with myocardial atrophy, compared with the model group. In the high dose lutein group, the exercise endurance increased, the cardiac index and left ventricular index [(3.02 卤0.28), (2.09 卤0.17) mg/g] decreased (P0.01), and the contents of HYP and malondialdehyde in myocardium [(0.28 卤0.06) g / mg, (1.89 卤0.61) nmol/mgpr, respectively] decreased (P0.05). The levels of SOD in serum [(72.70 卤571.19) U / mL, (571.19 卤15.58) U / mL] were significantly increased (P0.01), the interstitial components of myocardial tissue increased slightly, no inflammatory cells infiltrated, mild fibrous tissue hyperplasia and atrophic muscle atrophy were observed. Conclusion lutein has a protective effect on myocardial fibrosis induced by isoproterenol in rats, and its mechanism may be related to the improvement of antioxidant capacity of rats.
【作者单位】：吉林医药学院基础医学院;吉林医药学院临床医学院;
【基金】：吉林省教育厅“十二五”科学技术研究项目(2015396)
【分类号】：R151

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