天津某小区生活饮用水中胞外耐药基因污染分析

发布时间：2018-07-04 10:44

本文选题：生活饮用水 + 耐药基因　；参考：《军事医学》2017年04期

【摘要】：目的研究天津某小区生活饮用水中胞外耐药基因的污染特征,阐明生活饮用水中胞外耐药基因污染规律。方法于2015年12月至2016年11月,利用自行研制的核酸吸附柱富集生活饮用水中的游离核酸,采用实时荧光定量PCR技术检测tetC、ermB、ampC、sul1、qnrA、aadA、catA1和katG 8种胞外耐药基因的浓度。结果与结论在12份水样中,tet C的检出率最高,约为66.7%;其次为sul1和qnr A(41.7%)、ampC(33.3%)、aadA(16.7%)、cat A1和kat G(均为8.33%),erm B未检测出。生活饮用水中以sul1胞外耐药基因的污染最严重,达(1.27±0.04)×10~5GC/L。各胞外耐药基因出现最大浓度的时间主要集中在6月,且3月和6月检测出的胞外耐药基因种类最多,达5种。天津某小区生活饮用水中存在多种胞外耐药基因的污染。
[Abstract]:Objective to study the characteristics of extracellular drug resistance (ECR) gene contamination in drinking water of a small district in Tianjin, and to elucidate the regularity of extracellular drug resistance gene contamination in drinking water. Methods from December 2015 to November 2016, the free nucleic acid in drinking water was enriched by a self-made adsorption column of nucleic acid. The concentrations of eight extracellular drug resistant genes (ESRA 1 and katG) were detected by real-time fluorescence quantitative PCR. Results and conclusion in 12 water samples, the detection rate of tropotte C was the highest (about 66.7%), followed by sul1 and qnr A (41.7%). AAD A (33.3%) and kat G (8.33%) were not detected. The contamination of extracellular drug resistance (sul1) gene in drinking water was the most serious, reaching (1.27 卤0.04) 脳 10 ~ (5) GC / L. The time of maximum concentration of each extracellular resistance gene was mainly in 6 months, and the number of extracellular resistance genes detected in March and June was the highest, as many as 5. There are many extracellular drug resistance genes in drinking water of a small district in Tianjin.
【作者单位】：天津大学化工学院;军事医学科学院卫生学环境医学研究所天津市环境与食品安全风险监控技术重点实验室;
【基金】：国家自然科学基金面上项目(81372947)
【分类号】：R123

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