双酚A对发育期大鼠免疫器官及调节性T细胞功能影响的研究

发布时间：2018-07-18 15:07

【摘要】：目的探讨发育期大鼠暴露低剂量双酚A(BPA)对免疫器官及调节性T细胞功能的影响及机制。方法初断乳20只雄性大鼠按照体重随机分为2组,分别为对照组(含1%无水乙醇的水)和双酚A染毒组(1μg/ml),每组10只,通过饮水方式进行BPA染毒8周。实验结束后,计算大鼠胸腺系数和脾脏系数;ELISA方法检测大鼠血清肿瘤坏死因子-α(TNF-α)、干扰素γ(IFN-γ)和白细胞介素-4(IL-4)的含量;流式细胞术检测大鼠脾脏CD4+T细胞中调节性T细胞(Treg)数量和脾细胞线粒体膜电位。结果与对照组比较,BPA组(1μg/ml)大鼠胸腺系数和脾脏系数未见明显差异;血清TNF-α和IFN-γ水平明显升高(P0.01和P0.05),IL-4水平没有明显差异;脾脏细胞中Treg数量降低(P0.05),脾细胞线粒体膜电位下降(P0.05)。结论低剂量BPA(1μg/ml)暴露对发育期雄鼠的免疫器官脏器系数未见显著影响,但可影响Th1免疫应答;其机制可能与BPA增加机体炎症反应和诱导脾细胞凋亡有关。
[Abstract]:Objective to investigate the effect and mechanism of low dose bisphenol A (BPA) exposure on immune organs and regulatory T cell function in developing rats. Methods Twenty male rats were randomly divided into two groups according to their body weight: control group (water containing 1% anhydrous alcohol) and bisphenol A (1 渭 g/ml) group, 10 rats in each group were treated with BPA-treated by drinking water for 8 weeks. At the end of the experiment, the thymus and spleen coefficients were calculated and the serum levels of tumor necrosis factor- 伪 (TNF- 伪), interferon 纬 (IFN- 纬) and interleukin-4 (IL-4) were measured by Elisa. The number of regulatory T cells (Treg) in CD4 T cells and mitochondrial membrane potential of spleen cells were measured by flow cytometry. Results compared with the control group, there was no significant difference in thymus coefficient and spleen coefficient, the levels of serum TNF- 伪 and IFN- 纬 increased significantly (P0.01 and P0.05), and the number of Treg in spleen cells decreased (P0.05), and the mitochondrial membrane potential of spleen cells decreased (P0.05). Conclusion low dose BPA (1 渭 g/ml) exposure has no significant effect on the organ coefficient of immune organs, but can affect the Th1 immune response, and its mechanism may be related to the increase of inflammatory response and the induction of splenocyte apoptosis.
【作者单位】：中国医科大学附属盛京医院风湿免疫科;中国医科大学公共卫生学院儿少卫生学教研室;
【基金】：辽宁省自然科学基金(2015020466) 沈阳市科学技术项目(F14-158-9-44)
【分类号】：R114

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