胶原蛋白肽氨基酸组成对小鼠肠道吸收的影响研究

发布时间：2018-07-18 17:24

【摘要】：研究了胶原蛋白肽氨基酸组成对其在小鼠体内吸收程度的影响。利用离子交换层析分别制备出富含碱性和酸性氨基酸的胶原蛋白肽,小鼠灌胃后检测了血清中羟脯氨酸和胶原蛋白降解后的多肽相对丰度的差异。氨基酸组成分析结果表明阳离子交换层析制备的胶原蛋白肽(CPC)富含精氨酸(Arg)和赖氨酸(Lys),含量比未经过层析分离的多肽(CPo)分别高12.2%和7.4%。阴离子交换制备的多肽(CPA)中Arg和Lys含量比CPo低18.3%和26.5%。通过离子阱质谱技术从CPC中检测到9种含Arg或Lys的多肽,其含量远大于CPA中的含量。动物实验结果表明CPC在小鼠体内吸收高于CPA,小鼠血清中CPC在吸收峰值时Hyp的浓度是CPA的1.39倍,采用三重四级杆质谱(TSQ)从CPC灌胃后小鼠血清中检测出含Hyp的寡肽(Ser-Hyp-Gly、Pro-Hyp-Gly、Leu-Hyp),3种寡肽分别是CPA灌胃后小鼠血清中浓度的2.1、1.48和1.51倍,初步证明了氨基酸组成是影响胶原蛋白肽在小鼠体内吸收的重要因素。
[Abstract]:The effect of amino acid composition of collagen peptide on its absorption in mice was studied. Collagen peptides rich in alkaline and acidic amino acids were prepared by ion exchange chromatography. The relative abundance of serum hydroxyproline and collagen degraded peptides in mice were detected by gastric perfusion. The results of amino acid composition analysis showed that the collagen peptides prepared by cationic exchange chromatography were rich in arginine (Arg) and lysine, and the contents of them were 12.2% and 7.4% higher than those of unpurified polypeptides (CPOs), respectively. The contents of Arg and Lys in the polypeptides prepared by anion exchange were 18.3% and 26.5% lower than those in CPO. Nine kinds of polypeptides containing Arg or Lys were detected by ion trap mass spectrometry. The results of animal experiments showed that the absorption of CPC in mice was higher than that of CPA, and the concentration of Hyp in serum of mice was 1.39 times higher than that of CPA at the peak absorption of CPC. Three kinds of oligopeptides containing Hyp were detected by triple quadruple mass spectrometry (TSQ) from the serum of mice fed with CPC. The results showed that the three oligopeptides were 2.1 ~ 1.48 and 1.51 times higher than those of mice fed with CPA, respectively. The results showed that amino acid composition was an important factor affecting the absorption of collagen peptides in mice.
【作者单位】：山东农业大学食品科学与工程学院;中国科学院过程工程研究所生化工程重点实验室;
【基金】：国家自然科学基金(21306205) 863项目(2014AA022109)
【分类号】：TS201.4

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