铅暴露对大鼠海马组织APP和tau蛋白表达影响

发布时间：2018-07-27 13:39

【摘要】：目的探讨长期铅暴露对大鼠海马组织淀粉样前体蛋白(APP)和tau基因、蛋白表达影响。方法将SPF级雌性SD大鼠随机分为3组,分别为对照组(去离子水),低、高剂量铅组(乙酸铅800、1 500 mg/L),所生雄性仔鼠分别继续饮用去离子水(对照组),300 mg/L(低剂量铅组),900 mg/L(高剂量铅组)乙酸铅溶液,分别与生后3周(PNW3)、中年期(PNW41)、老年期(PNW70)测定雄性仔鼠血、海马组织中铅含量;采用荧光定量PCR(q PCR)和蛋白印迹(WB)检测APP和tau mRNA及蛋白表达水平。结果与对照组比较,各年龄染铅组雄性仔鼠血铅、海马铅含量明显增加(P0.05);与对照组比较,高剂量铅组PNW70雄性仔鼠海马APP mRNA和蛋白表达水平[分别为(1.398±0.827)、(1.454±0.190)]均明显上升(P0.05);与对照组比较,低、高剂量铅组PNW3雄性仔鼠海马tau蛋白表达水平[分别为(4.850±1.257)、(5.557±1.880)]明显上升(P0.01);对照组比较,高剂量铅组PNW70雄性仔鼠海马磷酸化tau蛋白表达水平(3.225±1.098)升高(P0.05)。结论长期铅暴露可导致断乳期和老年期大鼠海马组织APP和tau基因与蛋白表达上调。
[Abstract]:Objective to investigate the effects of long term lead exposure on the expression of amyloid precursor protein (APP) and amyloid precursor protein (tau) in hippocampus of rats. Methods female SD rats of SPF grade were randomly divided into three groups: the control group (deionized water), the control group (deionized water). In the high dose lead group (lead acetate 800 ~ 1 500 mg/L), the male offspring rats continued to drink deionized water (control group) for 300 mg/L (low dose lead group) and lead acetate solution (high dose lead group) for 3 weeks (PNW3), middle age (PNW41), and old age (PNW70) to determine the blood of male offspring, respectively, at 3 weeks after birth (PNW3), middle age (PNW41) and old age (PNW70). The levels of APP, tau mRNA and protein were detected by fluorescence quantitative PCR (q PCR) and Western blot (WB). Results compared with the control group, the blood lead and hippocampal lead content of the male rats exposed to lead in all ages were significantly increased (P0.05), and compared with the control group, The expression levels of APP mRNA and protein in hippocampus of PNW70 male rats in high dose lead group [(1.398 卤0.827), (1.454 卤0.190)] were significantly higher than those in control group [(4.850 卤1.257), (5.557 卤1.880)] (P 0.05), and significantly higher in high dose lead group than in control group (4.850 卤1.257), (5.557 卤1.880), compared with control group, the expression of tau protein in hippocampus of PNW3 male rats in high dose lead group was significantly higher than that in control group (P 0.05). The expression of phosphorylated tau protein in hippocampus of PNW70 male rats in high dose lead group (3.225 卤1.098) was increased (P0.05). Conclusion prolonged lead exposure may lead to up-regulation of APP and tau gene and protein expression in hippocampal tissues of weaning and aged rats.
【作者单位】：华中科技大学同济医院;南昌大学公共卫生学院;
【基金】：国家自然科学基金(30960324;21267017)
【分类号】：R114

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