丙烯酰胺对雌性Wistar大鼠学习记忆和长时程增强的影响及其可能机制
发布时间：2018-08-04 14:28
【摘要】：目的研究重复sc给予丙烯酰胺(AM)28 d后对学习记忆的影响及其可能的作用机制,以及单次sc给予AM后对大鼠海马高频刺激后群峰电位(PS)幅度改变的影响。方法雌性Wistar大鼠分别sc给予AM 10,20和40 mg·kg-1(给药当天计为第1天),连续给予28 d。每周记录1次体质量。于第22~第27天进行Morris水迷宫实验,评价AM对大鼠空间定位能力的影响;分别于第29和第30天进行跳台实验,观察5 min内大鼠跳下平台的次数以及首次跳下平台的时间。取AM 40 mg·kg-1组大鼠海马组织,Western蛋白印迹法检测N-甲基-D-天冬氨酸受体(NR)2A及2B与Ⅱ型钙调蛋白激酶(Ca MKⅡ)的表达与磷酸化水平。另取Wistar雌性大鼠,单次sc给予AM 40 mg·kg-1,进行长时程增强(LTP)实验,观察高频刺激后PS的改变。结果与正常对照组相比,给予AM后,大鼠体质量增长减缓(P0.01)。AM 20及40 mg·kg-1组大鼠找到平台的时间显著延长,但是对大鼠穿越平台的次数无显著影响。与实验首日(第29天)相比,经过电击强化学习记忆,正常对照组大鼠为躲避电击,次日(第30天)5 min内跳下平台的次数显著减少(P0.01),且首次跳下平台的时间明显延长(P0.01),而AM 10,20和40 mg·kg-1组5 min内的错误次数以及首次跳下平台的时间无显著改变。Western蛋白印迹结果显示,与正常对照组相比,AM 40 mg·kg-1组大鼠海马组织中NR2A及NR2B的表达与磷酸化水平显著增加,且Ca MKⅡ磷酸化水平显著升高(P0.01)。LTP结果显示,与正常对照组相比,单次sc给予AM 40 mg·kg-1显著降低Wistar雌性大鼠海马的PS幅度(P0.01),损伤了大鼠海马部位的突触可塑性。结论 AM可能通过改变谷氨酸的含量,增加NR的表达与活性,导致钙超载,引发神经细胞死亡,从而使LTP的PS幅度下降,影响海马神经突触的可塑性,最终影响中枢的学习记忆功能。
[Abstract]:Objective to study the effects of repeated acrylacrylamide (AM) on learning and memory and its possible mechanism, and the effects of single sc administration of AM on the changes of (PS) amplitude of group potential after high-frequency stimulation in hippocampus of rats. Methods female Wistar rats were given AM1020 and 40 mg kg-1 (day 1) for 28 days, respectively. Body mass was recorded once a week. The Morris water maze test was carried out from 22 to 27 days to evaluate the influence of AM on the spatial positioning ability of rats, and the platform jumping experiments were carried out on the 29th and 30th days, respectively, to observe the times of jumping off the platform and the time of the first jumping from the platform within 5 min. The expression and phosphorylation of N-methyl-Daspartate receptor (NR) 2A), calmodulin kinase type 2B (2B) and calmodulin kinase 鈪，
本文编号：2164171
[Abstract]:Objective to study the effects of repeated acrylacrylamide (AM) on learning and memory and its possible mechanism, and the effects of single sc administration of AM on the changes of (PS) amplitude of group potential after high-frequency stimulation in hippocampus of rats. Methods female Wistar rats were given AM1020 and 40 mg kg-1 (day 1) for 28 days, respectively. Body mass was recorded once a week. The Morris water maze test was carried out from 22 to 27 days to evaluate the influence of AM on the spatial positioning ability of rats, and the platform jumping experiments were carried out on the 29th and 30th days, respectively, to observe the times of jumping off the platform and the time of the first jumping from the platform within 5 min. The expression and phosphorylation of N-methyl-Daspartate receptor (NR) 2A), calmodulin kinase type 2B (2B) and calmodulin kinase 鈪，
本文编号：2164171
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