牛磺酸锌对铅暴露大鼠脂质过氧化损伤的拮抗作用

发布时间：2018-08-08 18:34

【摘要】：目的探讨牛磺酸锌(taurine-zinc,TZC)对铅暴露大鼠脂质过氧化损伤的拮抗作用。方法将40只3周龄健康雄性SD大鼠随机分成5组,分别为对照组、染铅组和10、50、100 mg/kg TZC拮抗组,每组8只。除对照组外,其余4组大鼠灌胃乙酸铅溶液(30 mg/kg)染毒;之前1 h,各剂量TZC拮抗组灌胃相应剂量的TZC溶液,每天1次,连续45 d。实验结束后测定各组大鼠血铅含量及血清中总抗氧化能力(T-AOC)、丙二醛(MDA)含量、超氧化物歧化酶(SOD)活力及抑制羟自由基能力。结果染铅组大鼠血铅含量和血清中MDA含量高于对照组,血清中T-AOC、SOD活力、抑制羟自由基能力低于对照组,差异有统计学意义(P0.05)。各剂量TZC拮抗组SOD活力、T-AOC和抑制羟自由基能力均介于对照组和染铅组之间;且随着TZC剂量的升高,染铅大鼠血清中MDA含量呈先下降后升高的趋势,血清中T-AOC、SOD活力及抑制羟自由基能力均呈先升高后下降的趋势,并均以50 mg/kg TZC拮抗组最接近对照组。结论牛磺酸锌可拮抗铅导致的大鼠脂质过氧化损伤。
[Abstract]:Objective to investigate the antagonistic effect of taurine-zincinine (TZC) on lipid peroxidation in rats exposed to lead. Methods Forty 3-week-old male Sprague-Dawley rats were randomly divided into five groups: control group, lead exposed group and 1050100 mg/kg TZC antagonist group with 8 rats in each group. In addition to the control group, rats in the other four groups were given lead acetate solution (30 mg/kg) by gastric administration, and the corresponding dose of TZC solution was injected into the stomach of each dose of TZC antagonist group for 45 days at the first hour. After the experiment, the contents of blood lead, total antioxidant capacity (T-AOC), malondialdehyde (MDA), superoxide dismutase (SOD) activity and the ability of inhibiting hydroxyl radical were measured. Results the contents of blood lead and MDA in serum of rats exposed to lead were higher than those of control group, and the activity of T-AOCU SOD and the ability of inhibiting hydroxyl free radical in serum were lower than those in control group (P0.05). The activity of SOD, T-AOC and the ability of inhibiting hydroxyl free radical in each dose of TZC antagonistic group were between control group and lead group, and with the increase of TZC dose, the content of MDA in serum of rats exposed to lead decreased first and then increased. The activity of T-AOC SOD and the ability of inhibiting hydroxyl free radical in serum increased first and then decreased, and the 50 mg/kg TZC antagonistic group was the closest to the control group. Conclusion Zinc taurine can antagonize the lipid peroxidation injury induced by lead in rats.
【作者单位】：滨州医学院烟台校区医药研究中心;
【分类号】：R151

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