小剂量玉米赤霉烯酮损害雄性小鼠生殖能力的研究

发布时间：2018-08-13 10:10

【摘要】：目的评估小剂量(低于无明显作用水平的浓度)玉米赤霉烯酮(zearalenone,ZEA)暴露后小鼠精子发生及精液质量的变化趋势,探讨小剂量ZEA暴露对雄性小鼠生殖能力的影响。方法 90只出生后21 d的雄性CD-1小鼠按随机数字表法分成3组,分别暴露于0、20、40μg/(kg·d)ZEA,处理14、28、42 d后,采用计算机辅助精液质量分析观察精子活力参数,吉姆萨染色观测精子畸形率,测量睾丸体积及质量,免疫组化染色检测生精细胞计数。结果处理42 d后,20、40μg/(kg·d)组精子活动率低于0μg/(kg·d)组[(82.80±4.34)%、(74.90±6.70)%vs(93.20±1.66)%,P=0.004、0.002];20、40μg/(kg·d)组小鼠畸形精子率高于0μg/(kg·d)组[(29.40±1.09)%、(40.80±6.17)%vs(19.40±0.45)%,P0.001,P=0.002];20、40μg/(kg·d)组小鼠的单位面积生精细胞计数低于0μg/(kg·d)组(2 422.7±206.1、1 855.8±105.3 vs 3 189.8±306.0,P=0.013、0.001)。结论小剂量ZEA的暴露能够降低雄性小鼠的精子发生及精液质量,损害其生殖能力。
[Abstract]:Objective To evaluate the changes of spermatogenesis and semen quality in mice exposed to zearalenone (ZEA) at low doses (below the concentration of no significant effect), and to explore the effects of low doses of ZEA on the reproductive ability of male mice. After exposed to 0,20,40 UG / (kg d) ZEA for 14,28,42 days, sperm motility parameters were observed by computer-assisted semen quality analysis, sperm deformity rate was observed by Jimsa staining, testicular volume and mass were measured, and spermatogenic cell count was detected by immunohistochemical staining. Results After 42 days of treatment, sperm motility in 20,40 UG / (kg d) group was lower than that in 0 UG / (kg d) group [(82. 80 [(80 [(74.90 [(74.90 [(6.70)%vs (93.90 [(93.20 [(1.66)%, P = 0.004, 0.002]; (20, 40 [(29.40 [1.09)%%, (40.80 [6.17)%vs (19.40 [19.40 [0.40 [0.45)%, (P = 0.001, P = 0.002]; (20, 40 UG /(kg /(d) micein 20, 40 UG [(kg / (kg /) d) had higher abspercountof spercells per unit area in 20, 40 UG / (kg / (kg / (kg / (d) day) group than 0 0 0 0 6.1,1 855.8+105.3 vs 3 18 Conclusion Low dose ZEA exposure can reduce spermatogenesis and semen quality of male mice, and impair their reproductive ability.
【作者单位】：第三军医大学大坪医院野战外科研究所泌尿外科;
【基金】：国家自然科学基金面上项目(31572225)~~
【分类号】：R114

【相似文献】