纳米硫化铅和醋酸铅对大鼠脑组织氨基酸类神经递质影响研究

发布时间：2018-08-13 10:58

【摘要】：目的探讨纳米硫化铅和醋酸铅亚慢性经口染毒对大鼠脑组织中谷氨酸(Glu)、丝氨酸(Ser)、甘氨酸(Gly)和γ-氨基丁酸(GABA)等氨基酸类神经递质的影响。方法无特定病原体级健康雌性SD大鼠随机分为对照组、醋酸铅组和纳米硫化铅组,每组15只。2个铅染毒组大鼠分别予给醋酸铅(100 mg/kg体质量)和纳米硫化铅混悬液(10 mg/kg体质量)灌胃,对照组予等体积质量分数为0.9%的氯化钠溶液灌胃,每周5次,连续12周。染毒结束处死大鼠,分离皮质和海马组织,采用电感耦合等离子体-质谱法测定铅水平,采用高效液相色谱法检测Glu、Ser、Gly和GABA水平。结果醋酸铅和纳米硫化铅组大鼠皮质、海马组织内的铅水平均分别高于对照组(P0.05);纳米硫化铅组大鼠皮质和海马组织中的铅水平均低于醋酸铅组(P0.05),分别为醋酸铅组的48.8%和54.0%。与对照组比较,醋酸铅组和纳米硫化铅组大鼠的皮质、海马组织中Glu水平均升高,GABA和Gly水平均下降(P0.05);纳米硫化铅组大鼠皮质组织中Ser水平升高(P0.05),而海马组织中Ser水平下降(P0.05)。与醋酸铅组比较,纳米硫化铅组大鼠皮质组织中Glu和Ser水平均升高(P0.05),海马组织中的GABA水平下降(P0.05)。结论纳米硫化铅和醋酸铅暴露均可导致大鼠脑组织内氨基酸类神经递质水平改变;纳米硫化铅暴露的影响较醋酸铅更为明显,这可能是其发挥神经毒性的毒理学机制之一。
[Abstract]:Objective to investigate the effects of nanometer lead sulfide and lead acetate on amino acid neurotransmitters such as glutamate (Glu), serine (Ser), glycine (Gly) and 纬 -aminobutyric acid (GABA) in rat brain. Methods healthy female SD rats without specific pathogens were randomly divided into control group, lead acetate group and nanometer lead sulfide group. 15 rats in each group were given lead acetate (100 mg/kg body mass) and nanometer lead sulfide suspension (10 mg/kg body mass) respectively. The control group was given sodium chloride solution with equal volume fraction of 0.9%, 5 times a week for 12 weeks. The rats were killed and the cortical and hippocampal tissues were isolated. The lead levels were determined by inductively coupled plasma-mass spectrometry (ICP-MS) and the levels of gly and GABA were detected by high performance liquid chromatography (HPLC). Results lead in cortex and hippocampus in lead acetate group and nanometer lead sulfide group were significantly higher than those in control group (P0.05), and lead levels in cortex and hippocampus of lead acetate group were lower than those in lead acetate group (P 0.05), which were 48.8% and 54.0% of those in lead acetate group, respectively. Compared with the control group, the levels of Glu and Gly in hippocampus of lead acetate group and nanometer lead sulfide group were all increased (P0.05), the level of Ser in cortex tissue increased (P0.05), and the Ser level in hippocampal tissue decreased (P0.05) in lead acetate group and nano-lead sulfide group (P0.05). Compared with lead acetate group, the levels of Glu and Ser in cortex were increased (P0.05) and the level of GABA in hippocampus was decreased (P0.05) in nano-lead sulfide group. Conclusion the level of amino acid neurotransmitters in rat brain can be changed after exposure to lead sulfide nanoparticles and lead acetate, which may be one of the toxicological mechanisms of its neurotoxicity.
【作者单位】：华北理工大学公共卫生学院;
【基金】：国家自然科学基金(81373033) 大学生创新性实验项目(X2013039) 华北理工大学培育基金(SP201511)
【分类号】：R135

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