苦荞醇溶粗提物对淀粉酶的影响及体内抗氧化性研究
发布时间：2018-08-13 11:29
【摘要】：苦荞富含以芦丁为主的黄酮类物质,与其他谷物相比有更高的食用价值和药用价值。由于快消化淀粉会产生较高的餐后血糖反应而导致氧化应激,对机体造成氧化损伤,因此本文首先以淀粉的消化率为切入点,探究了苦荞醇溶粗提物对α-淀粉酶活性的影响,进一步采用动物实验研究了苦荞醇溶粗提物通过其抗氧化活性对酒精性肝损伤的保护作用。采用70%的乙醇,料液比1:35(g/m L),在80℃下提取苦荞粉得到苦荞醇溶粗提物及提取后的苦荞残粉,粗提物得率为6.26%。通过薄层层析和超高效液相色谱串联四级飞行时间质谱法(UPLC-Q-TOF/MS)分析苦荞醇溶粗提物成分,可知其主要成分芦丁和槲皮素的含量分别为:198.89 mg/g粗提物,3.56 mg/g粗提物,此外还含有其他黄酮类物质如山柰酚-3-O-芸香糖苷、色素、大分子糖类和酚酸类物质等。通过对比苦荞粉和苦荞残粉的双酶水解消化曲线可知苦荞醇溶粗提物会显著减缓苦荞粉的消化。进一步探究影响淀粉代谢的原因,以抑制率、抑制类型、荧光猝灭效应实验来分析苦荞醇溶粗提物对α-淀粉酶的抑制作用,结果表明苦荞醇溶粗提物对α-淀粉酶的抑制作用与其浓度相关,IC50值为3.35 mg/m L,抑制类型为非竞争性抑制,并可使α-淀粉酶的荧光效应发生静态猝灭。小鼠的餐后血糖曲线也表明了苦荞醇溶粗提物可显著降低餐后血糖反应,影响了普通玉米淀粉在小鼠体内的消化吸收。以亚铁还原能力法(FRAP)确定了苦荞醇溶粗提物的抗氧化性后进行动物实验,采用高脂膳食和急性酒精饲喂C57BL/6雄性小鼠,探究苦荞醇溶粗提物的体内抗氧化作用。结果表明高脂饲料会显著促进小鼠增重,苦荞醇溶粗提物对小鼠脂代谢无显著影响;酒精处理(最后1周)后小鼠体重减轻,而苦荞醇溶粗提物能够降低体重减轻的幅度,表明对酒精损伤有一定保护作用。进而分析小鼠血清中丙氨酸转氨酶(ALT)和天冬氨酸转氨酶(AST)的活性,酒精处理后,他们的活性显著上升,说明肝功能受损,但饲料中的苦荞醇溶粗提物能有效抵抗ALT和AST水平的升高,也表明了苦荞醇溶粗提物对酒精肝损伤的保护作用。通过分析肝脏和小肠组织中的超氧化物歧化酶(SOD)活力、谷胱甘肽过氧化物酶(GSH-Px)活力与丙二醛(MDA)含量,结果表明高脂膳食和急性酒精处理会造成小鼠肝脏和小肠抗氧化能力的下降,出现氧化损伤,而苦荞醇溶粗提物有效提高了组织的抗氧化效应,小肠中的抗氧化性尤其显著。通过对比小鼠的肝脏和腹部脂肪组织切片可知,酒精处理会造成小鼠的肝脏受损,苦荞醇溶粗提物可缓和肝细胞间的炎性浸润现象,表明对肝脏有一定的保护作用,而粗提物的抗氧化活性可能是重要的机制之一,值得进一步探究。
[Abstract]:Tartary buckwheat is rich in rutin-based flavonoids and has higher edible and medicinal value than other cereals. Fast digestible starch can produce high postprandial blood glucose reaction and lead to oxidative stress, which can cause oxidative damage to body. Therefore, the effect of alcohol soluble crude extract of Tartary buckwheat on 伪 -amylase activity was studied by using starch digestibility as the starting point. The protective effect of Tartary buckwheat alcohol soluble crude extract on alcoholic liver injury through its antioxidant activity was further studied by animal experiments. Tartary buckwheat powder was extracted from Tartary buckwheat powder with 70% ethanol at 1:35 (g / m L),) at 80 鈩，
本文编号：2180848
[Abstract]:Tartary buckwheat is rich in rutin-based flavonoids and has higher edible and medicinal value than other cereals. Fast digestible starch can produce high postprandial blood glucose reaction and lead to oxidative stress, which can cause oxidative damage to body. Therefore, the effect of alcohol soluble crude extract of Tartary buckwheat on 伪 -amylase activity was studied by using starch digestibility as the starting point. The protective effect of Tartary buckwheat alcohol soluble crude extract on alcoholic liver injury through its antioxidant activity was further studied by animal experiments. Tartary buckwheat powder was extracted from Tartary buckwheat powder with 70% ethanol at 1:35 (g / m L),) at 80 鈩，
本文编号：2180848
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