焦炉工尿中多环芳烃代谢物、血浆BPDE-白蛋白加合物与外周血淋巴细胞DNA损伤的关联性研究

发布时间：2018-08-14 19:05

【摘要】：目的：研究PAHs暴露致工人早期外周血淋巴细胞DNA损伤情况，并进一步探讨尿多环芳烃代谢物、血浆BPDE-白蛋白加合物与DNA损伤之间的关系。方法：选取湖北省武汉市某焦化厂1167名男性工人作为研究对象，选取办公室、辅助车间、炉底和炉侧、炉顶等代表性工作岗位进行工作场所PAHs浓度的检测；对所有工人都进行职业健康体检；同时采用调查表的形式收集每个工人的人口学信息、吸烟饮酒史、既往史、家族遗传史以及职业暴露史；采集每个工人尿液，使用气相色谱-质谱联用仪（GC-MS）检测尿液中10种多环芳烃代谢物的含量，，评价工人职业性PAHs内暴露水平；酶联免疫吸附试验（ELISA）检测血浆BPDE-白蛋白浓度，评价PAHs暴露有效剂量水平；彗星试验检测外周血淋巴细胞DNA损伤程度，采用Olive尾矩（Olive tail moment, OTM）指标来评价PAHs致机体早期健康损害情况。将各代谢物四分位以分析其与DNA损伤之间剂量-效应关系。结果：除四羟基菲外其余九种尿多环芳烃代谢物、BPDE-白蛋白加合物及OTM均呈现办公室组＜辅助岗位组＜炉底和炉侧组＜炉顶组的趋势，且四组间差异具有统计学意义；Pearson相关分析发现1-羟基萘与所有OH-PAHs相关性最好，而1-羟基芘与BPDE-白蛋白相关性最好，除9-羟基芴、3-，4-羟基菲外其余七种尿OH-PAHs、BPDE-白蛋白加合物均与OTM呈正相关；以正相关的OH-PAHs浓度及BPDE-白蛋白加合物浓度四分位分组，都与OTM呈剂量-效应关系；1-羟基萘与BPDE-白蛋白加合物联合分析表明，与1-羟基萘第一组和BPDE-白蛋白加合物浓度最低组相比，1-羟基萘第四组和BPDE-白蛋白加合物浓度最高组DNA损伤水平升高有显著性（p＜0.001），而且与其他组相比升高的水平最多。结论：外周血淋巴细胞DNA损伤随职业性PAHs暴露水平增高而增加；尿7种OH-PAHs、血浆BPDE-白蛋白加合物与DNA损伤呈显著剂量-效应关系；1-羟基萘与BPDE-白蛋白加合物对PAHs暴露所造成的焦炉工外周血淋巴细胞DNA损伤具有联合作用。
[Abstract]:Objective: to study the DNA damage in peripheral blood lymphocytes of workers exposed to PAHs in early stage, and to explore the relationship between urinary polycyclic aromatic hydrocarbons metabolites, plasma BPDE-albumin adducts and DNA damage. Methods: 1167 male workers from a coking plant in Wuhan, Hubei Province were selected as the research objects. The PAHs concentration in the workplace was measured in representative work positions such as office, auxiliary workshop, furnace bottom, furnace side, furnace top and so on. All workers were examined for occupational health; demographics of each worker were collected in the form of questionnaires; smoking and drinking history, past history, family genetic history and occupational exposure history were collected; urine samples were collected from each worker. Ten polycyclic aromatic hydrocarbons (PAHs) metabolites in urine were detected by gas chromatography-mass spectrometry (GC-MS) to evaluate the exposure level of occupational PAHs in workers, the plasma BPDE- albumin concentration was detected by Elisa (ELISA), and the effective dose level of PAHs exposure was evaluated. Comet assay was used to detect the degree of DNA damage in peripheral blood lymphocytes and Olive tail moment (Olive tail moment, OTM) index was used to evaluate the early health damage caused by PAHs. Four quartiles of metabolites were used to analyze the dose-effect relationship between the metabolites and DNA damage. Results: nine urinary polycyclic aromatic hydrocarbons metabolites (BPDE- albumin adducts and OTM) except tetrahydroxy phenanthrene showed the trend of office group < auxiliary post group < furnace bottom group and furnace side group < furnace top group, and the differences among the four groups were statistically significant. Pearson correlation analysis showed that 1-hydroxynaphthalene had the best correlation with all OH-PAHs, while 1-hydroxypyrene had the best correlation with BPDE-albumin. The other seven urinary OH-PAHsBPDE-albumin adducts were positively correlated with OTM except 9-hydroxyfluorene 3-4-hydroxyphenanthrene. According to the positive correlation of OH-PAHs concentration and the concentration of BPDE- albumin adducts, there was a dose-effect relationship between BPDE-albumin adducts and 1-hydroxylnaphthalene and BPDE-albumin adducts. Compared with the first group of 1-hydroxynaphthalene and the lowest concentration group of BPDE-albumin adducts, the level of DNA damage in the fourth group and the highest concentration group was significantly increased (p < 0.001), and the highest level was found in the other groups. Conclusion: the DNA damage of peripheral blood lymphocytes increased with the increase of occupational PAHs exposure, and there was a significant dose-effect relationship between urinary seven OH-PAHs, plasma BPDE-albumin adducts and DNA damage. 1-hydroxynaphthalene and BPDE-albumin adducts have a combined effect on DNA damage in peripheral blood lymphocytes of coke oven workers caused by PAHs exposure.
【学位授予单位】：华中科技大学
【学位级别】：硕士
【学位授予年份】：2013
【分类号】：R131

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