酸性鞘磷脂酶在镉性肾损害中的变化
发布时间：2018-08-16 09:20
【摘要】：目的 观察酸性鞘磷脂酶(ASMase)在镉性肾损害过程中的活性变化及规律,探讨ASMase活性变化与镉性肾损害的关系。方法 60只SD雄性大鼠随机分为对照组和实验组,实验组动物腹腔注射1mg/kg Cd2+,每天1次、每周5次,对照组注射等体积的生理盐水。染毒第0、1、3、5和7周末实验组、对照组每组各随机处死6只动物,收集血、尿及肾组织。ELISA法测尿β2微球蛋白的含量,高效液相色谱法测血、尿及肾组织中ASMase活性,制作病理切片观察肾组织病变情况。结果 随染毒时间的延长,肾组织有不同程度的病理改变。与对照组相比,实验组动物肾脏系数随染毒时间的延长而增加(P0.05);尿β2微球蛋白含量随染毒时间的延长而增加(P0.05);血、尿、肾组织匀浆上清液中ASMase活性从染毒第3周开始随染毒时间的延长而增加(P0.05)。结论 镉性肾损害中ASMase活性随染毒时间的延长而增加。
[Abstract]:Objective to observe the activity and regularity of acid sphingolipase (ASMase) in the process of cadmium induced renal injury, and to explore the relationship between the activity of ASMase and cadmium induced renal damage. Methods Sixty Sprague-Dawley male rats were randomly divided into control group and experimental group. 1mg/kg Cd2 was injected intraperitoneally in the experimental group, once a day, 5 times a week, and the control group was injected with saline of the same volume. At the end of the 5th and 7th week of exposure, 6 animals were randomly killed in each group in the control group. The contents of 尾 2 microglobulin in urine, urine and renal tissue were collected by Elisa, and the activity of ASMase in blood, urine and renal tissue were measured by high performance liquid chromatography (HPLC). Pathological sections were made to observe the pathological changes of renal tissue. Results with the prolongation of exposure time, renal tissue had different pathological changes. Compared with the control group, the kidney coefficient of the experimental group increased with the time of exposure (P0.05); the content of urinary 尾 2 microglobulin increased with the prolongation of the exposure time (P0.05); the blood, urine, blood, urine, The activity of ASMase in the supernatant of renal homogenate increased with the prolongation of exposure time from the 3rd week of exposure (P0.05). Conclusion the activity of ASMase in cadmium induced renal injury increased with the prolongation of exposure time.
【作者单位】： 中南大学公共卫生学院卫生毒理学系;美国西奈山医学院基因与基因组学研究室;中南大学湘雅三医院病理科;
【分类号】：R994.6;;R114
[Abstract]:Objective to observe the activity and regularity of acid sphingolipase (ASMase) in the process of cadmium induced renal injury, and to explore the relationship between the activity of ASMase and cadmium induced renal damage. Methods Sixty Sprague-Dawley male rats were randomly divided into control group and experimental group. 1mg/kg Cd2 was injected intraperitoneally in the experimental group, once a day, 5 times a week, and the control group was injected with saline of the same volume. At the end of the 5th and 7th week of exposure, 6 animals were randomly killed in each group in the control group. The contents of 尾 2 microglobulin in urine, urine and renal tissue were collected by Elisa, and the activity of ASMase in blood, urine and renal tissue were measured by high performance liquid chromatography (HPLC). Pathological sections were made to observe the pathological changes of renal tissue. Results with the prolongation of exposure time, renal tissue had different pathological changes. Compared with the control group, the kidney coefficient of the experimental group increased with the time of exposure (P0.05); the content of urinary 尾 2 microglobulin increased with the prolongation of the exposure time (P0.05); the blood, urine, blood, urine, The activity of ASMase in the supernatant of renal homogenate increased with the prolongation of exposure time from the 3rd week of exposure (P0.05). Conclusion the activity of ASMase in cadmium induced renal injury increased with the prolongation of exposure time.
【作者单位】： 中南大学公共卫生学院卫生毒理学系;美国西奈山医学院基因与基因组学研究室;中南大学湘雅三医院病理科;
【分类号】：R994.6;;R114
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