迷迭香提取物对高脂高胆固醇膳食仓鼠小肠中胆固醇代谢调控机制的研究

发布时间：2018-08-22 12:21

【摘要】：目的研究迷迭香提取物对仓鼠血脂水平及小肠中胆固醇合成、吸收、代谢等基因调控的影响。方法以高脂高胆固醇膳食饲喂仓鼠为高脂血症模型,测定血清中总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白胆固醇(HDL-C)的含量,气相色谱法测定仓鼠肝脏中胆固醇和粪便中中性及酸性固醇的含量,Real time-PCR(RT-PCR)法检测小肠中HMG-Co A还原酶(HMGCR)、NPC1L1、ACAT2及ABCG5/8的表达水平。结果高脂高胆固醇(HF-HC)膳食造成高脂血症模型,迷迭香干预降低血清中TG含量(P0.05),且0.3%、0.9%剂量组HDL-C含量升高(P0.05);迷迭香组仓鼠肝脏中胆固醇含量较高脂高胆固醇组(HF-HC)显著降低(P0.01),且粪便中总中性及酸性固醇排出量较HF-HC组增加,0.3%、0.9%剂量组有统计学差异性(P0.05);RT-PCR结果显示,迷迭香可以下调胆固醇合成限速酶HMGCR及吸收关键酶NPC1L1基因的表达水平(P0.05)。结论迷迭香可通过升高HDL-C水平,增加固醇排泄调节高血脂仓鼠机体胆固醇平衡,其机制可能与抑制小肠中胆固醇合成、酯化、吸收相关酶的表达有关。
[Abstract]:Objective to study the effects of Rosemary extract on lipid level and cholesterol synthesis, absorption and metabolism in small intestine of hamsters. Methods the hyperlipidemia model of hamsters fed with high fat and high cholesterol diet was used to determine the content of serum total cholesterol (TC), triglyceride (TG), high density lipoprotein cholesterol (HDL-C). Determination of cholesterol and fecal Neutral and acidic steroids in the liver of Hamster by Gas Chromatography Real time-PCR (RT-PCR) method was used to detect the expression of HMG-Co A reductase (HMGCR) NPC1L1AACAT2 and ABCG5/8 in small intestine. Results the hyperlipidemia model was induced by hyperlipidemia (HF-HC) diet. Rosemary intervention decreased serum TG content (P0.05) and increased HDL-C content in 0.9% group (P0.05), while the liver cholesterol content in rosemary group was significantly lower than that in high-fat high-cholesterol group (HF-HC) (P0.01), and excretion of total neutral and acidic steroids in feces was higher than that in HF-HC group. The results of RT-PCR showed that there was a significant difference between the two groups (P0.05). Rosemary could down-regulate the expression of cholesterol synthesis rate-limiting enzyme HMGCR and absorption key enzyme NPC1L1 gene (P0.05). Conclusion Rosemary can increase the level of HDL-C and increase steroid excretion to regulate the cholesterol balance in hyperlipidemia hamsters, which may be related to the inhibition of cholesterol synthesis, esterification and the absorption of related enzymes in the small intestine.
【作者单位】：天津科技大学食品工程与生物技术学院;天津科技大学生物工程学院;
【基金】：国家自然科学基金(No.31201322) “十二五”国家科技计划(No.2012BAD33B05) 天津市高等学校科技发展基金计划(No.20100609) 天津科技大学大学生实验室创新基金项目(No.1314A216)
【分类号】：R151

【共引文献】