砷对HOXD10基因表达的影响
发布时间：2018-09-05 11:24
【摘要】：目的探讨砷对周围血淋巴细胞中及人肺腺癌细胞系A549细胞中同源异型盒D10(HOXD10)基因表达的影响。方法 (1)采用判断抽样方法,抽取某砒霜厂职业性接触砷的59名工人为接触组,以无职业性接触砷的17名当地居民为对照组。采用氢化物发生-冷肼捕集-原子吸收法检测2组人群尿中形态砷水平,采用实时荧光定量聚合酶链式反应(qRT-PCR)法检测周围血淋巴细胞中HOXD10的表达。(2)以浓度为0.0、0.1、0.5、1.0、2.0μmol/L的三氧化二砷(As_2O_3)处理A549细胞,采用比色法检测细胞存活率,采用qRT-PCR法检测HOXD10的表达。结果 (1)接触组人群尿中无机砷、一甲基砷酸、二甲基砷酸、总砷水平和周围血淋巴细胞中HOXD10 mRNA相对表达水平均高于对照组(P0.05)。(2)As_2O_3可导致A549细胞存活率呈现剂量依赖性下降(P0.01),HOXD10mRNA相对表达水平呈现剂量依赖性上调(P0.01)。A549细胞存活率和HOXD10 mRNA相对表达水平呈负相关,相关系数为-0.777(P0.01)。结论砷可导致职业接触人群周围血中HOXD10表达上调。As_2O_3可抑制A549细胞增殖,可能与上调HOXD10表达有关。
[Abstract]:Objective to investigate the effect of arsenic on the expression of homobox D10 (HOXD10) gene in peripheral blood lymphocytes and human lung adenocarcinoma cell line A549. Methods (1) 59 workers exposed to arsenic in a arsenic factory were selected as exposure group and 17 local residents without occupational exposure as control group. The levels of arsenic in urine were detected by hydride generation-cold hydrazine trapping and atomic absorption spectrometry. The expression of HOXD10 in peripheral blood lymphocytes was detected by real-time fluorescence quantitative polymerase chain reaction (qRT-PCR). (2) A549 cells were treated with arsenic trioxide (As_2O_3) at a concentration of 0. 01 渭 mol/L. The survival rate of A549 cells was detected by colorimetric assay and the expression of HOXD10 was detected by qRT-PCR assay. Results (1) Inorganic arsenic, monomethyl arsenic acid, dimethyl arsenic acid in urine of exposed group, The total arsenic level and the relative expression of HOXD10 mRNA in peripheral blood lymphocytes were higher than those in the control group (P0.05). (2) As_2O_3 could lead to a dose-dependent decrease in the survival rate of A549 cells (P0.01). The relative expression level of HOXD10 mRNA in A549 cells was up-regulated in a dose-dependent manner (P0.01). A549 cell survival rate and HOXD10 were increased in a dose-dependent manner. The relative expression level of mRNA was negatively correlated. The correlation coefficient was -0.777 (P0.01). Conclusion arsenic can induce the upregulation of HOXD10 expression in blood of occupational exposed population. As _ 2O _ 3 can inhibit the proliferation of A549 cells, which may be related to the up-regulation of HOXD10 expression.
【作者单位】： 昆明医科大学公共卫生学院;
【基金】：国家自然科学基金(81460491) 云南省公共卫生与疾病防控协同创新项目(2016YNPHXT15)
【分类号】：R135.1
[Abstract]:Objective to investigate the effect of arsenic on the expression of homobox D10 (HOXD10) gene in peripheral blood lymphocytes and human lung adenocarcinoma cell line A549. Methods (1) 59 workers exposed to arsenic in a arsenic factory were selected as exposure group and 17 local residents without occupational exposure as control group. The levels of arsenic in urine were detected by hydride generation-cold hydrazine trapping and atomic absorption spectrometry. The expression of HOXD10 in peripheral blood lymphocytes was detected by real-time fluorescence quantitative polymerase chain reaction (qRT-PCR). (2) A549 cells were treated with arsenic trioxide (As_2O_3) at a concentration of 0. 01 渭 mol/L. The survival rate of A549 cells was detected by colorimetric assay and the expression of HOXD10 was detected by qRT-PCR assay. Results (1) Inorganic arsenic, monomethyl arsenic acid, dimethyl arsenic acid in urine of exposed group, The total arsenic level and the relative expression of HOXD10 mRNA in peripheral blood lymphocytes were higher than those in the control group (P0.05). (2) As_2O_3 could lead to a dose-dependent decrease in the survival rate of A549 cells (P0.01). The relative expression level of HOXD10 mRNA in A549 cells was up-regulated in a dose-dependent manner (P0.01). A549 cell survival rate and HOXD10 were increased in a dose-dependent manner. The relative expression level of mRNA was negatively correlated. The correlation coefficient was -0.777 (P0.01). Conclusion arsenic can induce the upregulation of HOXD10 expression in blood of occupational exposed population. As _ 2O _ 3 can inhibit the proliferation of A549 cells, which may be related to the up-regulation of HOXD10 expression.
【作者单位】： 昆明医科大学公共卫生学院;
【基金】：国家自然科学基金(81460491) 云南省公共卫生与疾病防控协同创新项目(2016YNPHXT15)
【分类号】：R135.1
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